Neural Tissue Dissociation Kits

Name: Neural Tissue Dissociation Kits
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Neural Tissue Dissociation Kits

Product overview

The Neural Tissue Dissociation Kits have been developed for the gentle and efficient generation of single-cell suspensions from neural tissues.

The Neural Tissue Dissociation Kit - Postnatal Neurons has been optimized for the special needs of sensitive postnatal neurons during dissociation.

Product data and images for Neural Tissue Dissociation Kits

Figures

Figure 1

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Figure 1

Antigen compatibility of Dissociation Kits. For many applications, including MACS Cell Separation, it is crucial to conserve the integrity of particular cell surface epitopes during tissue dissociation. The table shows which kits conserve which specific epitopes. Epitope sensitivities have been tested using Miltenyi Biotec's antibodies if available.

Specifications for Neural Tissue Dissociation Kits

Overview

The Neural Tissue Dissociation Kits have been developed for the gentle and efficient generation of single-cell suspensions from neural tissues.

The Neural Tissue Dissociation Kit - Postnatal Neurons has been optimized for the special needs of sensitive postnatal neurons during dissociation.

Detailed product information

Background information

In less than an hour, the gentle two-step enzymatic dissociation procedure efficiently yields high numbers of viable cells. The Neural Tissue Dissociation Kit (P) contains papain, and the Neural Tissue Dissociation Kit (T) contains trypsin.

Some antigen epitopes are damaged by papain, others by trypsin. The kit based on papain is best suited for use with Anti-Prominin-1 MicroBeads, CD11b (Microglia) MicroBeads and Anti-A2B5 MicroBeads, whereas, for example, Anti-GLAST (ACSA-1) MicroBead Kit and Anti-PSA-NCAM MicroBeads require the use of the trypsin-based kit.

Please see figure 1 for further information with regard to epitope sensitivity and kit selection.

Resources for Neural Tissue Dissociation Kits

Documents and Protocols

Brochures/posters

Neuroscience - Inspiring technologies for creative scientists

Scientific posters

Semi-automated tissue dissociation and preserved epitope integrity optimize immunomagnetic sorting of neural cells

Scientific posters

Automated adult and neonatal mouse brain dissociation and magnetic isolation of neurons increases efficiency and sensitivity for single-cell RNA sequencing and gene expression profiling. Reiß, S. et al. SfN (2019)

Scientific posters

A novel approach for efficient dissociation of distinct adult mouse brain regions and subsequent sorting of neurons and neural stem cells using MACSQuant® Tyto®. Reiß, S. et al. (2015)

App notes/customer reports

Dissociation of mouse brain to isolate microglia

Protocols

Isolation of endothelial cells from mouse neonatal brain

Protocols

Dissection of retinas

Protocols

Schwann cell isolation from rat peripheral nerve tissue biopsies

Reviews for Neural Tissue Dissociation Kits

Quick and Easy Neural Tissue Dissociation Kit

Neural Tissue Dissociation Kit (P) (130-092-628)

I really enjoy using this kit to isolate neurons from mouse brain. The entire protocol is takes less than a couple hours and the instructions are easy to follow.

Best Kit for Embryonic and Postnatal Brain Tissue

Neural Tissue Dissociation Kit (P) (130-092-628)

This kit works very well for embryonic and postnatal rat brain tissue ( day 1 to day 7 rat tested). Compared to traditional trypsin-based method, this kit yields more total cells as well as viable cells.

Kit For Neuronal Tissue Processing

Neural Tissue Dissociation Kit (P) (130-092-628)

Our lab has developed different mouse models to study the efficacy of therapeutic candidates against HIV. One of the mouse lines we generated expresses human cell receptors which allows the cells to be infected with HIV. We use this mouse line to study HIV infection in the brain. We use this kit to process the brain tissue before we proceed to cell isolation.

Neural Tissue Dissociation Kit

Neural Tissue Dissociation Kit (P) (130-092-628)

We use this kit to dissociate mouse brain tissue to isolate microglia for in vitro experiments. The kit provides an excellent way to dissociate brain tissue to isolate cells of interest.

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References for Neural Tissue Dissociation Kits

Publications

Környei et al. (2007) Astroglia-derived retinoic acid is a key factor in glia-induced neurogenesis. FASEB J. 21: 2496-2509
Lee, J. K. et al. (2008) Regulator of G-protein signaling 10 promotes dopaminergic neuron survival via regulation of the microglial inflammatory response. J. Neurosci. 28: 8517-8528
Skog, J. et al. (2008) Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nat. Cell Biol. 10(12): 1470-1476
Szulwach, K. E. et al. (2010) Cross talk between microRNA and epigenetic regulation in adult neurogenesis. J. Cell Biol. 189: 127-141

Data and images