Clone:
REA470
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC

Extended validation for TCR Vγ9 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA470
B3++
Cells were incubated with an excess of purified unconjugated TCR Vγ9 (REA470) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using TCR Vγ9 (REA470). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using TCR Vγ9 (REA470). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using TCR Vγ9 (REA470). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for TCR Vγ9 Antibody, anti-human, REAfinity™

Overview

Clone REA470 recognizes the human variable gamma 9 subunit of the γ/δ T cell receptor (TCR Vγ9). The T cell receptor is a heterodimeric glycoprotein associated with the CD3 antigen. The γ and δ TCR chains are composed of constant and variable regions, each encoded by distinct gene segments. γδ T cells represent unconventional lymphocytes that share characteristics of natural killer (NK) cells and T cells and often express regulatory NK receptors, thereby linking innate and adaptive immunity. Upon cross-linking CD94/NKG2A, antigenic activation of γδ T cells is down-modulated by inhibiting cell proliferation, cytokine production, and cytotoxicity. γδ T cells can detect target cells with patterns of MHC class I expression altered as a result of tumor transformation or viral infection, thus endowing them with a role in immunosurveillance originally attributed to NK cells only. Vγ9/Vδ2 T cells comprise the majority of peripheral human γδ T cells and are capable of responding to the microbial metabolite HMB-PP.
Additional information: Clone REA470 displays negligible binding to Fc receptors.

Detailed product information

Technical specifications

CloneREA470
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
cynomolgus monkey (
Macaca fascicularis
)
,
chimpanzee (
Pan troglodytes
)
AntigenTCR Vγ9
Molecular mass of antigen [kDa]13
Distribution of antigenT cells
Entrez Gene ID6983
RRIDAB_2811563, AB_2857762, AB_2857750, AB_2857783, AB_2857774, AB_2653945, AB_2653946, AB_2653949, AB_2653950, AB_2811586

Resources for TCR Vγ9 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for TCR Vγ9 Antibody, anti-human, REAfinity™

Publications

  1. Eberl, M. et al. (2005) Human Vgamma9/Vdelta2 effector memory T cells express the killer cell lectin-like receptor G1 (KLRG1). J. Leukoc. Biol. 77(1): 67-70
  2. Karunakaran, M. M. et al. (2014)
    Vγ9 and Vδ2 T cell antigen receptor genes and butyrophilin 3 (BTN3) emerged with placental mammals and are concomitantly preserved in selected species like alpaca (
    Vicugna pacos
    ).
    Immunogenetics 66(4): 243-254
  3. Pár, G. et al. (2002)
    Decrease in CD3-negative-CD8
    dim+
    and Vdelta2/Vgamma9 TcR
    +
    peripheral blood lymphocyte counts, low perforin expression and the impairment of natural killer cell activity is associated with chronic hepatitis C virus infection.
    J. Hepatol. 37(4): 514-522

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