Clone:
REAL175
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
IF, MICS, IHC
Alternative names:
LY6A, Ly-6A.2, Ly-6A/E, Ly-6E.1, TAP

Specifications for Sca-1 Antibody, anti-mouse,
REAlease
®

Overview

Clone REAL175 is an antibody fragment derived from the full Sca-1 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL175 recognizes the mouse stem cell antigen-1 (Sca-1) antigen, an 18 kDa glycosylphosphatidylinositol (GPI) linked protein which is also known as lymphocyte antigen 6A and 6E (Ly-6A/E). Clone REAL175 specifically recognizes both Ly-6E.1 and Ly-6A.2, which are gene products of the two Ly-6A/E alleles expressed in different mouse strains (Ly-6E.1: e.g. BALB/c, C3H, NZB; Ly-6A.2: e.g. C57BL/6, SJL, 129, AKR). Sca-1 is expressed on hematopoietic stem cells and progenitor cells in mouse bone marrow and is one of the defining markers for so-called KSL (c-kit
+
Sca-1
+
Lin
) cells. In combination with CD105 (Endoglin) expression, Sca-1 has been used to define long-term repopulating hematopoietic stem cells (LTR-HSCs) in mouse bone marrow. In addition, Sca-1
+
bone marrow cells also contain the mesenchymal stem cell fraction. Sca-1
+
cells have been shown to give rise to hepatocytes
in vivo
and neural cells
in vitro
. Furthermore, Sca-1 is expressed on stem cells in a variety of nonhematopoietic tissues, such as adult liver, heart, and prostate.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

LY6A, Ly-6A.2, Ly-6A/E, Ly-6E.1, TAP

Detailed product information

Technical specifications

CloneREAL175
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Speciesmouse
AntigenSca-1
Alternative names of antigenLY6A, Ly-6A.2, Ly-6A/E, Ly-6E.1, TAP
Distribution of antigenhematopoietic stem and progenitor cells
RRIDAB_2857540, AB_2784451, AB_2857424, AB_2751265

Resources for Sca-1 Antibody, anti-mouse,
REAlease
®

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Sca-1 Antibody, anti-mouse,
REAlease
®

Publications

  1. Khan, K. D. et al. (1990) Characterization of promoter elements of an interferon-inducible Ly-6E/A differentiation antigen, which is expressed on activated T cells and hematopoietic stem cells. Mol. Cell. Biol. 10(10): 5150-5159
  2. Bradfute, S. B. et al. (2005) Roles of Sca-1 in hematopoietic stem/progenitor cell function. Exp. Hematol. 33(7): 836-843
  3. Houlihan, D. D. et al. (2012) Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-α. Nat. Protoc. 7(12): 2103-2111

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REAlease
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