Plasmacytoid Dendritic Cell Isolation Kit II, human

Plasmacytoid Dendritic Cell Isolation Kit II, human

The Plasmacytoid Dendritic Cell Isolation Kit II allows the isolation of untouched human plasmacytoid dendritic cells (PDCs) from PBMCs. Having evolved from the first-generation kit (# 130-092-207), it now offers even better cell purity and yield in a faster procedure.

Data and images
Specifications

Product data and images for Plasmacytoid Dendritic Cell Isolation Kit II, human

Figures

Figure 1

Human PDCs were isolated from PBMCs by using the Plasmacytoid Dendritic Cell Isolation Kit II, an LD Column, and a MidiMACS™ Separator. Cells were stained with CD303 (BDCA-2)-APC and CD123-PE before or after separation and analyzed by flow cytometry using the MACSQuant Analyzer.

PBMCs before separation	Enriched PDCs
View details Plasmacytoid Dendritic Cell Isolation Kit II, human Figure 1 Human PDCs were isolated from PBMCs by using the Plasmacytoid Dendritic Cell Isolation Kit II, an LD Column, and a MidiMACS™ Separator. Cells were stained with CD303 (BDCA-2)-APC and CD123-PE before or after separation and analyzed by flow cytometry using the MACSQuant Analyzer.	View details Plasmacytoid Dendritic Cell Isolation Kit II, human Figure 1 Human PDCs were isolated from PBMCs by using the Plasmacytoid Dendritic Cell Isolation Kit II, an LD Column, and a MidiMACS™ Separator. Cells were stained with CD303 (BDCA-2)-APC and CD123-PE before or after separation and analyzed by flow cytometry using the MACSQuant Analyzer.

Specifications for Plasmacytoid Dendritic Cell Isolation Kit II, human

Overview

The Plasmacytoid Dendritic Cell Isolation Kit II allows the isolation of untouched human plasmacytoid dendritic cells (PDCs) from PBMCs. Having evolved from the first-generation kit (# 130-092-207), it now offers even better cell purity and yield in a faster procedure.

Detailed product information

Background information

The isolated PDCs are CD303 (BDCA-2)

⁺

, CD304 (BDCA-4/Neuropilin-1)

⁺

, CD123

⁺

, CD4

⁺

, CD45RA

⁺

, CD141 (BDCA-3)

^dim

, CD1c (BDCA-1)

^–

, and CD2

^–

. The cells lack expression of lineage markers (CD3, CD14, CD16, CD19, CD20, CD56) and express neither myeloid markers, such as CD13 and CD33, nor Fc receptors, such as CD16, CD64, or FcεRI.

Detailed separation procedure

PDCs are isolated by depletion of non-PDCs. To this end, non-PDCs are magnetically labeled by using cocktails containing specific MicroBeads, biotin-conjugated antibodies, and Anti-Biotin MicroBeads. Magnetically labeled cells are retained in the column, while unlabeled PDCs with high purity are collected in the flow-through.

Downstream applications

PDCs isolated from human PBMCs can be used for phenotypic, functional, and molecular analyses, e.g., studies on expression of Toll-like receptors, chemokine receptors, or new antigens, and on DC activation, migration, cytokine production, and T cell polarization.

The Plasmacytoid Dendritic Cell Isolation Kit can also be used to enrich PDCs from tonsils, if the CD303 (BDCA-2) MicroBead Kit is not suitable.

Columns

LS, LD, or autoMACS

^®

Columns.

Data and images

Data and images for Plasmacytoid Dendritic Cell Isolation Kit II, human

Figures

Figure 1

Human PDCs were isolated from PBMCs by using the Plasmacytoid Dendritic Cell Isolation Kit II, an LD Column, and a MidiMACS™ Separator. Cells were stained with CD303 (BDCA-2)-APC and CD123-PE before or after separation and analyzed by flow cytometry using the MACSQuant Analyzer.

PBMCs before separation	Enriched PDCs
View details Plasmacytoid Dendritic Cell Isolation Kit II, human Figure 1 Human PDCs were isolated from PBMCs by using the Plasmacytoid Dendritic Cell Isolation Kit II, an LD Column, and a MidiMACS™ Separator. Cells were stained with CD303 (BDCA-2)-APC and CD123-PE before or after separation and analyzed by flow cytometry using the MACSQuant Analyzer.	View details Plasmacytoid Dendritic Cell Isolation Kit II, human Figure 1 Human PDCs were isolated from PBMCs by using the Plasmacytoid Dendritic Cell Isolation Kit II, an LD Column, and a MidiMACS™ Separator. Cells were stained with CD303 (BDCA-2)-APC and CD123-PE before or after separation and analyzed by flow cytometry using the MACSQuant Analyzer.

Specifications

Specifications for Plasmacytoid Dendritic Cell Isolation Kit II, human

Overview

The Plasmacytoid Dendritic Cell Isolation Kit II allows the isolation of untouched human plasmacytoid dendritic cells (PDCs) from PBMCs. Having evolved from the first-generation kit (# 130-092-207), it now offers even better cell purity and yield in a faster procedure.

Detailed product information

Background information

The isolated PDCs are CD303 (BDCA-2)

⁺

, CD304 (BDCA-4/Neuropilin-1)

⁺

, CD123

⁺

, CD4

⁺

, CD45RA

⁺

, CD141 (BDCA-3)

^dim

, CD1c (BDCA-1)

^–

, and CD2

^–

. The cells lack expression of lineage markers (CD3, CD14, CD16, CD19, CD20, CD56) and express neither myeloid markers, such as CD13 and CD33, nor Fc receptors, such as CD16, CD64, or FcεRI.

Detailed separation procedure

PDCs are isolated by depletion of non-PDCs. To this end, non-PDCs are magnetically labeled by using cocktails containing specific MicroBeads, biotin-conjugated antibodies, and Anti-Biotin MicroBeads. Magnetically labeled cells are retained in the column, while unlabeled PDCs with high purity are collected in the flow-through.

Downstream applications

PDCs isolated from human PBMCs can be used for phenotypic, functional, and molecular analyses, e.g., studies on expression of Toll-like receptors, chemokine receptors, or new antigens, and on DC activation, migration, cytokine production, and T cell polarization.

The Plasmacytoid Dendritic Cell Isolation Kit can also be used to enrich PDCs from tonsils, if the CD303 (BDCA-2) MicroBead Kit is not suitable.

Columns

LS, LD, or autoMACS

^®

Columns.

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Plasmacytoid Dendritic Cell Isolation Kit II, human

Plasmacytoid Dendritic Cell Isolation Kit II, human

Product data and images for Plasmacytoid Dendritic Cell Isolation Kit II, human

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Figure 1

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Figure 1

Specifications for Plasmacytoid Dendritic Cell Isolation Kit II, human

Overview

Detailed product information

Background information

Detailed separation procedure

Downstream applications

Columns

Data and images

Data and images for Plasmacytoid Dendritic Cell Isolation Kit II, human

Figures

Figure 1

Figure 1

Figure 1

Specifications

Specifications for Plasmacytoid Dendritic Cell Isolation Kit II, human

Overview

Detailed product information

Background information

Detailed separation procedure

Downstream applications

Columns

Related products for
Plasmacytoid Dendritic Cell Isolation Kit II, human

5 products available | view all view less

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Plasmacytoid Dendritic Cell Isolation Kit II, human

Plasmacytoid Dendritic Cell Isolation Kit II, human

Product data and images for Plasmacytoid Dendritic Cell Isolation Kit II, human

Figures

Figure 1