Name: PCNA Antibody, anti-human/mouse/rat, REAfinity™
Availability: InStock

Intracellular flow cytometry

Conjugate:	APC
Recommended dilution:	1:50
Remark:	The antibody is suited for staining of formaldehyde-fixed cells.
Tested:	QC tested
Products used:	130-114-704, 130-114-513

MICS (MACSima Imaging Cyclic Staining)

Conjugate:	APC
Recommended dilution:	1:50
Fixation:	FFPE
Tested:	during development
Products used:	130-114-704, 130-114-513
Compatible applications:	IF, IHC

Specifications for PCNA Antibody, anti-human/mouse/rat, REAfinity™

Overview

Clone REA858 recognizes the human, mouse, and rat proliferating cell nuclear antigen (PCNA), a 36 kDa nuclear protein also known as cyclin or auxiliary protein of DNA polymerase delta. PCNA is expressed in the nucleus of all proliferating cells. PCNA plays a role in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Furthermore, it is involved in DNA damage response by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.

Additional information: Clone REA858 displays negligible binding to Fc receptors.

Alternative names

proliferating-cell-nuclear-antigen, Cyclin

Detailed product information

Technical specifications

Clone	REA858
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (I), human IgG1
Host	cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human, mouse, rat, non-human primate, other
Cross-reactivity	cynomolgus monkey ( Macaca fascicularis ) , rhesus monkey ( Macaca mulatta ) , chicken, rabbit, hamster, horse, sheep, dog, cat, ferret, xenopus
Antigen	PCNA
Alternative names of antigen	proliferating-cell-nuclear-antigen, Cyclin
Molecular mass of antigen [kDa]	29(same molecular weight for human and mouse/rat)
Entrez Gene ID	5111, 18538, 25737
RRID	AB_2726679, AB_2726764, AB_2726680, AB_2726765, AB_2726681, AB_2726766, AB_2726682, AB_2726763

References for PCNA Antibody, anti-human/mouse/rat, REAfinity™

Publications

Landberg, G. et al. (1990) Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and Ki-67 antigen: a new view of the cell cycle. Exp. Cell Res. 187(1): 111-118
Guzińska-Ustymowicz, K. et al. (2009) Correlation between proliferation markers: PCNA, Ki-67, MCM-2 and antiapoptotic protein Bcl-2 in colorectal cancer. Anticancer Res. 29(8): 3049-3052
Cazzalini, O. et al. (2014) CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis. Nucleic Acids Res. 42(13): 8433-8448
Ortega, J. et al. (2015) Phosphorylation of PCNA by EGFR inhibits mismatch repair and promotes misincorporation during DNA synthesis. Proc. Natl. Acad. Sci. U.S.A. 112(18): 5667-5672

Applications

Specifications

Specifications for PCNA Antibody, anti-human/mouse/rat, REAfinity™

Overview

Additional information: Clone REA858 displays negligible binding to Fc receptors.

Alternative names

proliferating-cell-nuclear-antigen, Cyclin

Detailed product information

Technical specifications

Clone	REA858
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (I), human IgG1
Host	cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human, mouse, rat, non-human primate, other
Cross-reactivity	cynomolgus monkey ( Macaca fascicularis ) , rhesus monkey ( Macaca mulatta ) , chicken, rabbit, hamster, horse, sheep, dog, cat, ferret, xenopus
Antigen	PCNA
Alternative names of antigen	proliferating-cell-nuclear-antigen, Cyclin
Molecular mass of antigen [kDa]	29(same molecular weight for human and mouse/rat)
Entrez Gene ID	5111, 18538, 25737
RRID	AB_2726679, AB_2726764, AB_2726680, AB_2726765, AB_2726681, AB_2726766, AB_2726682, AB_2726763

References

References for PCNA Antibody, anti-human/mouse/rat, REAfinity™

Publications

Landberg, G. et al. (1990) Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and Ki-67 antigen: a new view of the cell cycle. Exp. Cell Res. 187(1): 111-118
Guzińska-Ustymowicz, K. et al. (2009) Correlation between proliferation markers: PCNA, Ki-67, MCM-2 and antiapoptotic protein Bcl-2 in colorectal cancer. Anticancer Res. 29(8): 3049-3052
Cazzalini, O. et al. (2014) CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis. Nucleic Acids Res. 42(13): 8433-8448
Ortega, J. et al. (2015) Phosphorylation of PCNA by EGFR inhibits mismatch repair and promotes misincorporation during DNA synthesis. Proc. Natl. Acad. Sci. U.S.A. 112(18): 5667-5672

PCNA Antibody, anti-human/mouse/rat, REAfinity™

PCNA Antibody, anti-human/mouse/rat, REAfinity™

Intracellular flow cytometry

MICS (MACSima Imaging Cyclic Staining)

Specifications for PCNA Antibody, anti-human/mouse/rat, REAfinity™

Overview

Alternative names

Detailed product information

Technical specifications

References for PCNA Antibody, anti-human/mouse/rat, REAfinity™

Publications

Applications

Specifications

Specifications for PCNA Antibody, anti-human/mouse/rat, REAfinity™

Overview

Alternative names

Detailed product information

Technical specifications

References

References for PCNA Antibody, anti-human/mouse/rat, REAfinity™

Publications

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PCNA Antibody, anti-human/mouse/rat, REAfinity™

PCNA Antibody, anti-human/mouse/rat, REAfinity™

Intracellular flow cytometry

MICS (MACSima Imaging Cyclic Staining)

Specifications for PCNA Antibody, anti-human/mouse/rat, REAfinity™

Overview

Alternative names

Detailed product information

Technical specifications

References for PCNA Antibody, anti-human/mouse/rat, REAfinity™

Publications

Applications

Specifications

Specifications for PCNA Antibody, anti-human/mouse/rat, REAfinity™

Overview

Alternative names

Detailed product information

Technical specifications

References

References for PCNA Antibody, anti-human/mouse/rat, REAfinity™

Publications

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