Name: p53 acK382 Antibody, anti-human, REAfinity™
Availability: InStock

Intracellular flow cytometry

Conjugate:	PE
Recommended dilution:	1:11
Remark:	The antibody is suited for staining of formaldehyde-fixed cells.
Tested:	QC tested
Products used:	130-109-072, 130-109-017

Specifications for p53 acK382 Antibody, anti-human, REAfinity™

Overview

Clone REA529 recognizes the human cellular tumor antigen p53 acetylated in the C-terminal region at lysine 382 (acK382). The p53 protein is an important regulator of cell growth and, thus, functions as a tumor suppressor in many tumor types. The p53 tumor suppressor gene is altered or deleted in most solid human tumors. It is tightly regulated and plays a critical role in the cellular response to environmental stress. Upon a cell's exposure to stress, such as hypoxia or genomic damage, the p53 protein is expressed at high levels and is post-translationally modified. These modifications, which include phosphorylation, acetylation, and glycosylation occur rapidly and lead to the activation of p53, resulting in either G1 growth arrest or programmed cell death. The acetylation levels of p53 are significantly enhanced in response to stress and correlate well with p53 activation and stabilization. p53 acetylation at K382 plays an important role in depsipeptide-induced p21(Waf1/Cip1) expression.

Additional information: Clone REA529 displays negligible binding to Fc receptors.

Alternative names

TP53, BCC7, LFS1, TRP53, NY-CO-13

Detailed product information

Technical specifications

Clone	REA529
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (I), human IgG1
Host	cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human
Antigen	p53 acK382
Alternative names of antigen	TP53, BCC7, LFS1, TRP53, NY-CO-13
Molecular mass of antigen [kDa]	44
Distribution of antigen	other
Entrez Gene ID	7157
RRID	AB_2653129, AB_2653130, AB_2653131, AB_2653128

References for p53 acK382 Antibody, anti-human, REAfinity™

Publications

Zakut-Houri, R. et al. (1985) Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4(5): 1251-1255
Tang, Y. et al. (2008) Acetylation is indispensable for p53 activation. Cell 133(4): 612-626
Zhao, Y. et al. (2006) Acetylation of p53 at lysine 373/382 by the histone deacetylase inhibitor depsipeptide induces expression of p21(Waf1/Cip1). Mol. Cell. Biol. 26(7): 2782-2790

Applications

Specifications

Specifications for p53 acK382 Antibody, anti-human, REAfinity™

Overview

Additional information: Clone REA529 displays negligible binding to Fc receptors.

Alternative names

TP53, BCC7, LFS1, TRP53, NY-CO-13

Detailed product information

Technical specifications

Clone	REA529
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (I), human IgG1
Host	cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human
Antigen	p53 acK382
Alternative names of antigen	TP53, BCC7, LFS1, TRP53, NY-CO-13
Molecular mass of antigen [kDa]	44
Distribution of antigen	other
Entrez Gene ID	7157
RRID	AB_2653129, AB_2653130, AB_2653131, AB_2653128

References

References for p53 acK382 Antibody, anti-human, REAfinity™

Publications

Zakut-Houri, R. et al. (1985) Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4(5): 1251-1255
Tang, Y. et al. (2008) Acetylation is indispensable for p53 activation. Cell 133(4): 612-626
Zhao, Y. et al. (2006) Acetylation of p53 at lysine 373/382 by the histone deacetylase inhibitor depsipeptide induces expression of p21(Waf1/Cip1). Mol. Cell. Biol. 26(7): 2782-2790

p53 acK382 Antibody, anti-human, REAfinity™

p53 acK382 Antibody, anti-human, REAfinity™

Intracellular flow cytometry

Specifications for p53 acK382 Antibody, anti-human, REAfinity™

Overview

Alternative names

Detailed product information

Technical specifications

References for p53 acK382 Antibody, anti-human, REAfinity™

Publications

Applications

Specifications

Specifications for p53 acK382 Antibody, anti-human, REAfinity™

Overview

Alternative names

Detailed product information

Technical specifications

References

References for p53 acK382 Antibody, anti-human, REAfinity™

Publications

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p53 acK382 Antibody, anti-human, REAfinity™

p53 acK382 Antibody, anti-human, REAfinity™

Intracellular flow cytometry

Specifications for p53 acK382 Antibody, anti-human, REAfinity™

Overview

Alternative names

Detailed product information

Technical specifications

References for p53 acK382 Antibody, anti-human, REAfinity™

Publications

Applications

Specifications

Specifications for p53 acK382 Antibody, anti-human, REAfinity™

Overview

Alternative names

Detailed product information

Technical specifications

References

References for p53 acK382 Antibody, anti-human, REAfinity™

Publications

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