The NK Cell Activation/Expansion Kit is designed to activate and expand human NK cells. The kit consists of Anti-Biotin MACSiBead™ Particles and biotinylated antibodies against human CD335 (NKp46) and CD2. Anti-Biotin MACSiBead Particles loaded with biotinylated antibodies are used to activate and expand resting NK cells purified from human blood or PBMCs.

Data and images for NK Cell Activation/Expansion Kit, human

Figures

Figure 1

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Anti-Biotin MACSiBead Particles were loaded with CD335 (NKp46) and CD2 antibodies. NK cells were activated and expanded from PBMCs using one loaded MACSiBead particle per viable cell. Cells were cultured in NK MACS Medium supplemented with 5% AB serum and 500 IU/mL IL-2. After 2 weeks, NK cells were incubated with K562 target cells in different ratios.
In addition, NK cells incubated in NK MACS Medium supplemented with 5% AB serum and 500 IU/mL IL-2 alone were analyzed. Specific target cell lysis by NK cells was determined after 4 hours.

Figure 1

Anti-Biotin MACSiBead Particles were loaded with CD335 (NKp46) and CD2 antibodies. NK cells were activated and expanded from PBMCs using one loaded MACSiBead particle per viable cell. Cells were cultured in NK MACS Medium supplemented with 5% AB serum and 500 IU/mL IL-2. After 2 weeks, NK cells were incubated with K562 target cells in different ratios.
In addition, NK cells incubated in NK MACS Medium supplemented with 5% AB serum and 500 IU/mL IL-2 alone were analyzed. Specific target cell lysis by NK cells was determined after 4 hours.

Figure 2

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NK cell expansion starting with NK cells after separation with the NK Cell Isolation Kit (n=2). Cells were stimulated at day 1 using the NK Cell Activation/Expansion Kit, unstimulated cells were cultured in medium with IL‑2 alone.

Figure 2

NK cell expansion starting with NK cells after separation with the NK Cell Isolation Kit (n=2). Cells were stimulated at day 1 using the NK Cell Activation/Expansion Kit, unstimulated cells were cultured in medium with IL‑2 alone.

Figure 3

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NK cell Cytotoxicity Assay after 14 d culture, starting with PBMC. Cells were stimulated using the NK Cell Activation/Expansion Kit, unstimulated cells were cultured in medium with IL‑2 alone. Data are mean of duplicates.

Figure 3

NK cell Cytotoxicity Assay after 14 d culture, starting with PBMC. Cells were stimulated using the NK Cell Activation/Expansion Kit, unstimulated cells were cultured in medium with IL‑2 alone. Data are mean of duplicates.

Specifications for NK Cell Activation/Expansion Kit, human

Overview

The NK Cell Activation/Expansion Kit is designed to activate and expand human NK cells. The kit consists of Anti-Biotin MACSiBead™ Particles and biotinylated antibodies against human CD335 (NKp46) and CD2. Anti-Biotin MACSiBead Particles loaded with biotinylated antibodies are used to activate and expand resting NK cells purified from human blood or PBMCs.

Detailed product information

Downstream applications

NK cells, expanded by using antibody-loaded MACSiBead Particles, can be used for many downstream applications, for example, cytokine analysis, cytolytic activity, gene expression analysis, or functional studies.
1

References for NK Cell Activation/Expansion Kit, human

Publications

  1. Reim et al. (2009)
    Immunoselection of breast and ovarian cancer cells with trastuzumab and natural killer cells: selective escape of CD44
    high
    /CD24
    low
    /HER2
    low
    breast cancer stem cells.
    Cancer Res. 69: 8058-8066

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