Name: Neonatal Cardiac Fibroblast Isolation Kit, rat
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Rat neonatal hearts were dissociated using the Neonatal Heart Dissociation Kit, mouse and rat (# 130-098-373) in combination with the gentleMACS Octo Dissociator with Heaters. Subsequently, neonatal cardiac fibroblasts were isolated using the Cardiac Fibroblast Isolation Kit, rat and cultured in DMEM medium with 10 FBS and 100 U/mL penicillin/100 U/mL streptomycin for 1 day.

Immunofluorescence pictures show labeling of cardiac fibroblasts with an anti-Prolyl 4-hydroxylase β antibody (green) before (A) and after (B) magnetic enrichment using the Neonatal Cardiac Fibroblast Isolation Kit, rat. Nuclei are visualized by DAPI staining (blue).

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The Neonatal Cardiac Fibroblast Isolation Kit, rat has been designed for the enrichment of cardiac fibroblasts from dissociated neonatal rat hearts. The isolation of cardiac fibroblasts has been successfully tested using neonatal rat hearts from postnatal day 0 to day 3 (P0– P3).

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Cardiac fibroblasts are one of the most frequent cell types within the heart and play a critical role (i) in heart development, (ii) in maintaining normal cardiac function, as well as (iii) in cardiac remodeling during pathological conditions such as myocardial infarction. Moreover, cardiac fibroblasts are believed to be an ideal target population to be reprogrammed into cardiomyocyte-like cells for regenerative purposes. The Neonatal Cardiac Fibroblast Isolation Kit, rat has been designed for the enrichment of cardiac fibroblasts from dissociated neonatal rat hearts. The isolation of cardiac fibroblasts has been tested using neonatal rat hearts from postnatal day 0 to day 3 (P0–3). For optimal results, the Neonatal Cardiac Fibroblast Isolation Kit should be used in combination with the Neonatal Heart Dissociation Kit, mouse and rat (# 130-098-373).

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Neonatal Cardiac Fibroblast Isolation Kit, rat

Neonatal Cardiac Fibroblast Isolation Kit, rat

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Neonatal Cardiac Fibroblast Isolation Kit, rat

Neonatal Cardiac Fibroblast Isolation Kit, rat

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