IL-4 stands for interleukin 4. Mouse IL-4 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Data and images for Mouse IL-4

Figures

Figure 1

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Mouse IL-4 activity assay:
The specific activity is determined by proliferation assay using mouse HT-2 cells. Activity of Mouse IL-4, premium grade (red line) was compared to another commercially available product (black line) with equivalent outcome.

Figure 1

Mouse IL-4 activity assay:
The specific activity is determined by proliferation assay using mouse HT-2 cells. Activity of Mouse IL-4, premium grade (red line) was compared to another commercially available product (black line) with equivalent outcome.

Figure 2

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SDS-PAGE of Mouse IL-4, premium grade under reducing (R) and non-reducing (NR) conditions.

Figure 2

SDS-PAGE of Mouse IL-4, premium grade under reducing (R) and non-reducing (NR) conditions.

Figure 3

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Mouse IL-4 biological activity.
Activity of Mouse IL-4, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 3

Mouse IL-4 biological activity.
Activity of Mouse IL-4, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 4

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Mass spectrometry analysis (ESI-MS) of Mouse IL-4, premium grade. The peak corresponds to the calculated molecular mass of 13683 Da.

Figure 4

Mass spectrometry analysis (ESI-MS) of Mouse IL-4, premium grade. The peak corresponds to the calculated molecular mass of 13683 Da.

Specifications for Mouse IL-4

Overview

IL-4 stands for interleukin 4. Mouse IL-4 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse IL-4 can be used for a variety of applications, including:
  • In vitro differentiation of naive CD4+ T cells towards TH2 cells.
  • In vitro studies of bone marrow precursor development to DCs, together with GM-CSF.
  • In vitro investigation of B cell-T cell interaction, together with CD40-Ligand.

Detailed product information

Background information

IL-4 is produced mainly by activated T
H
2 cells and, to a lesser extent, by T
H
1 cells. It binds to the IL-4 receptor α (CD124), followed by the dimerization with other receptor chains to generate type 1 and type 2 receptors. IL-4 promotes the proliferation and differentiation of activated B cells and the expression of MHC class II antigens. Mice over expressing IL-4 have elevated levels of IgE and IgG1 and show a deficiency in T cell maturation. CD4
+
T cells from knockout mice lacking IL-4 are not able to produce T
H
2 cytokines after
in vitro
stimulation. The biological activity of IL-4 is species-specific, i.e. murine IL-4 is inactive on human cells and
vice versa.

Biological activity

  • Proliferation of HT-2 cells (NIBSC 91/656)
  • premium grade: ≥ 8×
    10
    6
    U/mg
  • research grade: ≥ 5×
    10
    6
    U/mg

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Mouse IL-4

Documents and Protocols

Background information

Premium-grade cytokine benefits

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

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