Clone:
REA1057
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
CAK1, MPF, MSLN

Extended validation for Mesothelin Antibody, anti-human, REAfinity™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Mesothelin. Capan-2 cells were stained with Mesothelin antibodies and plotted against the side scatter. As a control, Mesothelin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Mesothelin. Capan-2 cells were stained with Mesothelin antibodies and plotted against the side scatter. As a control, Mesothelin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Mesothelin. Capan-2 cells were stained with Mesothelin antibodies and plotted against the side scatter. As a control, Mesothelin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Mesothelin. Capan-2 cells were stained with Mesothelin antibodies and plotted against the side scatter. As a control, Mesothelin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Mesothelin. Capan-2 cells were stained with Mesothelin antibodies and plotted against the side scatter. As a control, Mesothelin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using Mesothelin (REA1057). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using Mesothelin (REA1057). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using Mesothelin (REA1057). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for Mesothelin Antibody, anti-human, REAfinity™

Overview

Clone REA1057 recognizes the human mesothelin antigen, a membrane glycoprotein also known as CAK1 or pre-pro-megakaryocyte-potentiating factor. Mesothelin is expressed on mesothelial cells and overexpressed on different tumors, e.g., mesotheliomas and adenocarcinomas. Mesothelin is a precursor protein which has functions in cellular adhesion.
Additional information: Clone REA1057 displays negligible binding to Fc receptors.

Alternative names

CAK1, MPF, MSLN

Detailed product information

Technical specifications

CloneREA1057
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenMesothelin
Alternative names of antigenCAK1, MPF, MSLN
Molecular mass of antigen [kDa]35
Distribution of antigentumor cells, other
Entrez Gene ID10232
RRIDAB_2733681, AB_2733876, AB_2733877, AB_2733435, AB_2733436, AB_2733558, AB_2733559, AB_2733680

Resources for Mesothelin Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Mesothelin Antibody, anti-human, REAfinity™

Publications

  1. Iwahori, K. et al. (2008) Megakaryocyte potentiating factor as a tumor marker of malignant pleural mesothelioma: evaluation in comparison with mesothelin. Lung Cancer 62(1): 45-54

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