The MACSxpress
®
Buffy Coat B Cell Isolation Kit was developed for the isolation of untouched B cells from buffy coat without density gradient centrifugation.

Data and images for
MACSxpress
®
Buffy Coat Pan B Cell Isolation Kit
, human

Figures

Figure 1

Untouched pan B cells were isolated from a buffy coat using the MACSxpress
®
Buffy Coat Pan B Cell Isolation Kit, a MACSmix™ Tube Rotator, and MACSxpress Separators. The isolated cells were fluorescently stained with CD19-PE, CD20-APC, and CD45-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
View details

Figure 1

Untouched pan B cells were isolated from a buffy coat using the MACSxpress
®
Buffy Coat Pan B Cell Isolation Kit, a MACSmix™ Tube Rotator, and MACSxpress Separators. The isolated cells were fluorescently stained with CD19-PE, CD20-APC, and CD45-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
View details

Figure 1

Untouched pan B cells were isolated from a buffy coat using the MACSxpress
®
Buffy Coat Pan B Cell Isolation Kit, a MACSmix™ Tube Rotator, and MACSxpress Separators. The isolated cells were fluorescently stained with CD19-PE, CD20-APC, and CD45-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Specifications for
MACSxpress
®
Buffy Coat Pan B Cell Isolation Kit
, human

Overview

The MACSxpress
®
Buffy Coat B Cell Isolation Kit was developed for the isolation of untouched B cells from buffy coat without density gradient centrifugation.

Detailed product information

Detailed separation procedure

With the new MACSxpress
®
Technology, untouched human leukocyte subsets can be isolated from up to 30 mL of buffy coat. Non-target cells are removed by immunomagnetic depletion using MACSxpress Beads. Simultaneously, erythrocytes are sedimented, yielding untouched target cells of high purity.

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®
Buffy Coat Pan B Cell Isolation Kit
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