MACSxpress
^®
Buffy Coat CD4 T Cell Isolation Kit
, human

Name: MACSxpress® Buffy Coat CD4 T Cell Isolation Kit, human
Availability: InStock

MACSxpress
^®
Buffy Coat CD4 T Cell Isolation Kit
, human

The MACSxpress

^®

Buffy Coat CD4 T Cell Isolation Kit was developed for the isolation of untouched CD4 T cells from buffy coat without density gradient centrifugation.

Data and images
Specifications

Product data and images for MACSxpress
^®
Buffy Coat CD4 T Cell Isolation Kit
, human

Figures

Figure 1

Untouched CD4 T cells were isolated from 30 mL of buffy coat using the MACSxpress

^®

Buffy Coat CD4 T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD3-FITC, CD4- PE, and CD45-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant Analyzer

^®

10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Figure 1

Untouched CD4 T cells were isolated from 30 mL of buffy coat using the MACSxpress

^®

Buffy Coat CD4 T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD3-FITC, CD4- PE, and CD45-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant Analyzer

^®

10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Figure 1

Untouched CD4 T cells were isolated from 30 mL of buffy coat using the MACSxpress

^®

Buffy Coat CD4 T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD3-FITC, CD4- PE, and CD45-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant Analyzer

^®

10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Specifications for MACSxpress
^®
Buffy Coat CD4 T Cell Isolation Kit
, human

Overview

The MACSxpress

^®

Buffy Coat CD4 T Cell Isolation Kit was developed for the isolation of untouched CD4 T cells from buffy coat without density gradient centrifugation.

Detailed product information

Detailed separation procedure

With the new MACSxpress

^®

Technology, untouched human leukocyte subsets can be isolated from up to 30 mL of buffy coat. Non-target cells are removed by immunomagnetic depletion using MACSxpress Beads. Simultaneously, erythrocytes are sedimented, yielding untouched target cells of high purity.

Data and images

Data and images for MACSxpress
^®
Buffy Coat CD4 T Cell Isolation Kit
, human

Figures

Figure 1

Untouched CD4 T cells were isolated from 30 mL of buffy coat using the MACSxpress

^®

Buffy Coat CD4 T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD3-FITC, CD4- PE, and CD45-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant Analyzer

^®

10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Figure 1

Untouched CD4 T cells were isolated from 30 mL of buffy coat using the MACSxpress

^®

Buffy Coat CD4 T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD3-FITC, CD4- PE, and CD45-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant Analyzer

^®

10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Figure 1

Untouched CD4 T cells were isolated from 30 mL of buffy coat using the MACSxpress

^®

Buffy Coat CD4 T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD3-FITC, CD4- PE, and CD45-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant Analyzer

^®

10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

Specifications

Specifications for MACSxpress
^®
Buffy Coat CD4 T Cell Isolation Kit
, human

Overview

The MACSxpress

^®

Buffy Coat CD4 T Cell Isolation Kit was developed for the isolation of untouched CD4 T cells from buffy coat without density gradient centrifugation.

Detailed product information

Detailed separation procedure

With the new MACSxpress

^®

Technology, untouched human leukocyte subsets can be isolated from up to 30 mL of buffy coat. Non-target cells are removed by immunomagnetic depletion using MACSxpress Beads. Simultaneously, erythrocytes are sedimented, yielding untouched target cells of high purity.

MACSxpress
^®
Buffy Coat CD4 T Cell Isolation Kit
, human

MACSxpress
^®
Buffy Coat CD4 T Cell Isolation Kit
, human

Product data and images for MACSxpress
^®
Buffy Coat CD4 T Cell Isolation Kit
, human

Figures

Figure 1

Figure 1

Figure 1

Specifications for MACSxpress
^®
Buffy Coat CD4 T Cell Isolation Kit
, human

Overview

Detailed product information

Detailed separation procedure

Data and images

Data and images for MACSxpress
^®
Buffy Coat CD4 T Cell Isolation Kit
, human

Figures

Figure 1

Figure 1

Figure 1

Specifications

Specifications for MACSxpress
^®
Buffy Coat CD4 T Cell Isolation Kit
, human

Overview

Detailed product information

Detailed separation procedure

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MACSxpress® Buffy Coat CD4 T Cell Isolation Kit, human

MACSxpress® Buffy Coat CD4 T Cell Isolation Kit, human

Product data and images for MACSxpress® Buffy Coat CD4 T Cell Isolation Kit, human

Figures

Figure 1