Clone:
TRFK-5
Type of antibody:
Primary antibodies, Functional-grade antibodies
Isotype:
rat IgG1, rat IgG1κ
Applications:
FA, ICFC

Specifications for IL-5 Antibody, anti-mouse

Overview

Interleukin 5 (IL-5) is pre-dominantly secreted by CD4
+
T cells. It is involved in a range of allergic reactions and mediates immune reactions against parasites. IL-5 also acts on other cell types such as B cells.
The Anti-IL-5 antibody has been designed for intracellular staining of IL-5–producing cells. Cells can be stimulated for IL-5-production, for example, by polyclonal stimulation with mitogens. For induction of IL-5 production by antigen-specific T cells, cells are restimulated with respective antigen. IL-5 can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IL-5–producing cells can be stained intracellularly with Anti-IL-5 antibodies. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IL-5–producing cells. Magnetically enriched cells can be stained intracellularly for IL-5 production directly on the MACS
®
Column. This procedure ensures higher sensitivity of detection and minimizes loss of cells during washing procedures. The protocol is useful for cytokine analysis of rare cells, for example, CD4
+
T cells in HIV patients, or other cell sources than peripheral blood mononuclear cells (PBMCs), e.g., bronchoalveolar lavages or synovial fluids.

Detailed product information

Technical specifications

CloneTRFK-5
Clonalitymonoclonal
Isotyperat IgG1κ, rat IgG1
Isotype controlIsotype Control Antibody, rat IgG1
Hostrat
Type of antibodyPrimary antibodies, Functional-grade antibodies
Speciesmouse
AntigenIL-5
Molecular mass of antigen [kDa]13
Entrez Gene ID16191
RRIDAB_2660015, AB_2660016, AB_10829953

Resources for IL-5 Antibody, anti-mouse

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

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IL-5 Antibody, anti-mouse

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