The IFN-α Secretion Assay – Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human IFN-α–secreting cells.

Data and images for IFN-α Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Figures

Figure 1

Human PBMCs were incubated with CpG ODN 2216 for 5 hours. Cells were stained and isolated according to secretion of IFN-α using the IFN-α Secretion Assay – Cell Enrichment and Detection Kit (PE) and CD303 (BDCA-2)-APC, and analyzed by flow cytometry.
Before enrichment
After enrichment
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Figure 1

Human PBMCs were incubated with CpG ODN 2216 for 5 hours. Cells were stained and isolated according to secretion of IFN-α using the IFN-α Secretion Assay – Cell Enrichment and Detection Kit (PE) and CD303 (BDCA-2)-APC, and analyzed by flow cytometry.
View details

Figure 1

Human PBMCs were incubated with CpG ODN 2216 for 5 hours. Cells were stained and isolated according to secretion of IFN-α using the IFN-α Secretion Assay – Cell Enrichment and Detection Kit (PE) and CD303 (BDCA-2)-APC, and analyzed by flow cytometry.

Specifications for IFN-α Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Overview

The IFN-α Secretion Assay – Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human IFN-α–secreting cells.

Detailed product information

Background information

Type I interferons (IFNs), including IFN-α, are a family of cytokines that have pleiotropic functions in the immune system.
1
Besides its potent antiviral activity, IFN-α exerts a variety of immunomodulatory effects, including positive and negative regulation of its own production, regulation of IL-12 and IFN-γ production, promotion of natural killer (NK) cell cytotoxicity, activation and maturation of dendritic cells, and the promotion of B cell development towards plasma cells.
2,3
IFN-α is expressed predominantly by CD123
+
CD11c
CD303 (BDCA2)
+
plasmacytoid dendritic cells, also referred to as natural IFN–producing cells. These cells, representing approximately 0.4% of total PBMCs, produce up to 1000-fold more type I IFNs than any other cell type. However, biologically relevant levels of IFN-α are also produced by other cell types, e.g., monocytes and myeloid dendritic cells.
4

Downstream applications

The kit can be used for the detection and enrichment of IFN-α–secreting cells for enumeration and phenotypic analysis as well as for culturing and functional characterization. Furthermore, the stimulatory or regulatory functions of cytokine-secreting monocytes and dendritic cells can be analysed.

Columns

MS, LS, or autoMACS
®
Columns.

References for IFN-α Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Publications

  1. Biron, C. A. (2001) Immunity 14: 661-664
  2. Montoya, M. et al. (2002) Type I interferons produced by dendritic cells promote their phenotypic and functional activation. Blood 99: 3263-3271
  3. Santini, S. M. et al. (2002) The natural alliance between type I interferon and dendritic cells and its role in linking innate and adaptive immunity. Proc. Natl. Acad. Sci. U.S.A. 22: 1071-1080
  4. Theofilopoulos, A. N. et al. (2005) Type I interferons (alpha/beta) in immunity and autoimmunity. Annu. Rev. Immunol. 23: 307-336

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