Alternative names:
IFN-γ

Data and images for Human IFN-γ1b

Figures

Figure 1

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Human IFN-γ1b activity assay.
The biological activity of Human IFN-γ1b is determined by inhibition assay using HT-29 cells. Activity of Human IFN-γ1b, premium grade (red line) was compared to another commercially available product (black line).

Figure 1

Human IFN-γ1b activity assay.
The biological activity of Human IFN-γ1b is determined by inhibition assay using HT-29 cells. Activity of Human IFN-γ1b, premium grade (red line) was compared to another commercially available product (black line).

Figure 2

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SDS-PAGE of Human IFN-γ1b, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Human IFN-γ1b, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

View details
Human IFN-γ1b biological activity.
Activity of Human IFN-γ1b, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 3

Human IFN-γ1b biological activity.
Activity of Human IFN-γ1b, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 4

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Mass spectrometry analysis (ESI-MS) of Human IFN-γ1b, premium grade. The peak corresponds to the calculated molecular mass of 16334 Da.

Figure 4

Mass spectrometry analysis (ESI-MS) of Human IFN-γ1b, premium grade. The peak corresponds to the calculated molecular mass of 16334 Da.

Specifications for Human IFN-γ1b

Overview

IFN-γ stands for interferon γ. Human IFN-γ is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IFN-γ1b can be used for a variety of applications, including:
  • Stimulation of monocytes, macrophages, and microglia.
  • In vitro maturation of Mo-DCs.
  • Apoptosis and cell growth inhibition assays.
  • Adhesion and migration assays.

Alternative names

IFN-γ

Detailed product information

Background information

IFN-γ, the only type II interferon, possesses potent anti-viral activity and immunoregulatory properties. It is structurally unrelated to the type I interferons and binds to a different receptor expressed by most immune cells. IFN-γ is produced predominantly by NK cells and T cells. IFN-γ enhances NK cell and macrophage activity and regulates B cell functions, like immunglobulin production and class switching. In addition, IFN-γ orchestrates leukocyte attraction and directs growth, maturation, and differentiation of many cell types. The activity of human IFN-γ is species-specific.

Biological activity

  • Proliferation of HT-29 cells (NIAID Gxg01-902-535)
  • premium grade: ≥ 2×
    10
    7
    IU/mg
    (typical activity: ≥ 5×
    10
    7
    IU/mg
    )
  • research grade: ≥ 1×
    10
    7
    IU/mg

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human IFN-γ1b

Documents and Protocols

Background information

Premium-grade cytokine benefits

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human IFN-γ1b

Publications

  1. Wong, V.L. et al. (1989) Growth-inhibitory activity of interferon-beta against human colorectal carcinoma cell lines. Int. J. Cancer 43: 526-530

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