CytoBox Mo-DC is a combination of two recombinant proteins optimized for use in cell culture, and differentiation studies.
CytoBox Mo-DC can be used for:
  • Differentiation of human blood-derived monocytes into human immature dendritic cells (Mo-DC)

Data and images for CytoBox Mo-DC, human

Figures

Figure 1

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Human IL-4 activity assay.
The biological activity of Human IL-4, premium grade was determined by proliferation assay using TF-1 cells.

Figure 1

Human IL-4 activity assay.
The biological activity of Human IL-4, premium grade was determined by proliferation assay using TF-1 cells.

Figure 2

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Human GM-CSF activity assay.
The biological activity of Human GM-CSF, premium grade was determined by proliferation assay using TF-1 cells. Activity of Human GM-CSF, premium grade, (red line) was compared to another commercially available product (black line).

Figure 2

Human GM-CSF activity assay.
The biological activity of Human GM-CSF, premium grade was determined by proliferation assay using TF-1 cells. Activity of Human GM-CSF, premium grade, (red line) was compared to another commercially available product (black line).

Specifications for CytoBox Mo-DC, human

Overview

CytoBox Mo-DC is a combination of two recombinant proteins optimized for use in cell culture, and differentiation studies.
CytoBox Mo-DC can be used for:
  • Differentiation of human blood-derived monocytes into human immature dendritic cells (Mo-DC)

Detailed product information

Background information

Dendritic cells (DCs) are the primary professional antigen presenting cells of the immune system. They have a central function for the initiation, programming and regulation of antigen-specific immune responses. In vitro, human DCs are often generated from peripheral blood monocytes using GM-CSF and IL-4. These monocyte-derived DCs (Mo-DCs) have the characteristics of immature DCs. Mature Mo-DCs can be obtained by culturing Mo-DCs in the presence of microbial, pro-inflammatory, or T cell-derived stimuli. Mo-DCs have been tested in a broad range of immunotherapy-based protocols, primarily for cancer research.

Biological activity

  • Proliferation of TF-1 cells (human GM-CSF NIBSC code 88/646, human IL-4 NIBSC 88/656).
  • premium grade: ≥ 5×
    10
    6
    IU/mg

Quality description

Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for CytoBox Mo-DC, human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

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