Clone:
REA627
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, 2610028K12Rik, E430033M20Rik, Mtvr1

Extended validation for CD71 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA627
C2++
RI7217++
Cells were incubated with an excess of purified unconjugated CD71 (REA627) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD71. C57BL/6 Mouse bone marrow were stained with CD71 antibodies and with a suitable counterstaining. As a control, CD71 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD71. C57BL/6 Mouse bone marrow were stained with CD71 antibodies and with a suitable counterstaining. As a control, CD71 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD71. C57BL/6 Mouse bone marrow were stained with CD71 antibodies and with a suitable counterstaining. As a control, CD71 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD71. C57BL/6 Mouse bone marrow were stained with CD71 antibodies and with a suitable counterstaining. As a control, CD71 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD71 (REA627). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD71 (REA627). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD71 (REA627). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD71 Antibody, anti-mouse, REAfinity™

Overview

Clone REA627 recognizes the mouse CD71 cell-surface antigen, which is also known as transferrin receptor protein 1 (TfR1), and essential for iron transport into proliferating cells. This homodimer is a single-pass type II membrane protein, which is able to bind one transferrin molecule per 90 kDa subunit. CD71 plays a role in cell proliferation by controlling the supply of iron and is expressed on erythroid precursors, immature proliferating cells, reticulocytes, but is very low expressed on resting leukocytes.
Additional information: Clone REA627 displays negligible binding to Fc receptors.

Alternative names

Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, 2610028K12Rik, E430033M20Rik, Mtvr1

Detailed product information

Technical specifications

CloneREA627
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD71
Alternative names of antigenTransferrin receptor protein 1, TR, TfR, TfR1, Trfr, 2610028K12Rik, E430033M20Rik, Mtvr1
Molecular mass of antigen [kDa]86
Distribution of antigenleukocytes, hematopoietic stem and progenitor cells
Entrez Gene ID22042
RRIDAB_2659130, AB_2659131, AB_2659132, AB_2659133, AB_2659136, AB_2659137, AB_2905114, AB_2751587, AB_2784273, AB_2751633, AB_2784274

Resources for CD71 Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD71 Antibody, anti-mouse, REAfinity™

Publications

  1. Lesley, J. et al. (1984) Expression of transferrin receptor on murine hematopoietic progenitors. Cell. Immunol. 83(1): 14-25
  2. Koulnis, M. et al. (2011) Identification and analysis of mouse erythroid progenitors using the CD71/TER119 flow-cytometric assay. J. Vis. Exp. (54)
  3. Levy, J. E. et al. (1999) Transferrin receptor is necessary for development of erythrocytes and the nervous system. Nat. Genet. 21(4): 396-399

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