Clone:
REA292
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
CD27L, CD27LG, TNFSF7

Extended validation for CD70 Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD70 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD70-PE (REA292, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD70 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD70-PE (REA292, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD70 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD70-PE (REA292, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD70 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD70-PE, clone (REA292). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD70 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD70-PE, clone (REA292). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD70 Antibody, anti-human, REAfinity™

Overview

Clone REA292 recognizes the human CD70 antigen, a 50 kDa type II transmembrane glycoprotein also known as CD27 ligand (CD27L) or tumor necrosis factor ligand superfamily member 7 (TNFSF7). CD70 is a member of the TNF superfamily and is expressed by activated mouse T, B, and NK lymphocytes, activated plasmacytoid dendritic cells (pDCs), chronic B cell lymphocytic leukemia, and large B cell lymphomas. It interacts with CD27, its ligand on T cells, in order to promote T-B cell cross-stimulation and co-stimulates B cell proliferation and immunoglobulin production. It also plays a role in the pDC-induced B cell differentiation.
Additional information: Clone REA292 displays negligible binding to Fc receptors.

Alternative names

CD27L, CD27LG, TNFSF7

Detailed product information

Technical specifications

CloneREA292
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD70
Alternative names of antigenCD27L, CD27LG, TNFSF7
Molecular mass of antigen [kDa]21
Distribution of antigenB cells, dendritic cells, NK cells, T cells
Entrez Gene ID970
RRIDAB_2811409, AB_2659112, AB_2659113, AB_2659116, AB_2659117, AB_2659118, AB_2659119, AB_2659120, AB_2659121, AB_2659122, AB_2659123, AB_2659124, AB_2659125, AB_2811421

Resources for CD70 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD70 Antibody, anti-human, REAfinity™

Publications

  1. Bowman, M. R. et al. (1994) The cloning of CD70 and its identification as the ligand for CD27. J. Immunol. 152(4): 1756-1761
  2. Goodwin, R. G. et al. (1993) Molecular and biological characterization of a ligand for CD27 defines a new family of cytokines with homology to tumor necrosis factor. Cell 73(3): 447-456

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