Clone:
REA994
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
T3

Extended validation for CD3 Antibody, anti-non-human primate, REAfinity™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD3. Rhesus monkey peripheral blood mononuclear cells (PBMCs) were stained with CD3antibodies and with a suitable counterstaining. As a control, CD3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD3. Rhesus monkey peripheral blood mononuclear cells (PBMCs) were stained with CD3antibodies and with a suitable counterstaining. As a control, CD3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD3. Rhesus monkey peripheral blood mononuclear cells (PBMCs) were stained with CD3antibodies and with a suitable counterstaining. As a control, CD3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD3. Rhesus monkey peripheral blood mononuclear cells (PBMCs) were stained with CD3antibodies and with a suitable counterstaining. As a control, CD3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD3 (REA994). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD3 (REA994). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD3 (REA994). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD3 Antibody, anti-non-human primate, REAfinity™

Overview

Clone REA994 recognizes rhesus monkey (
Macaca mulatta
) and cynomolgus monkey (
Macaca fascicularis
) CD3 T cells. The antibody has been reported to be cross-reactive with pigtail monkey (
Macaca nemestrina
), olive baboon monkey (
Papio anubis
), and african green monkey (
Chlorocebus aethiops
) T cells. CD3 is expressed on all T cells and on a subset of NK cells. It is associated with the T cell receptor (TCR) and is responsible for its signal transduction.
Additional information: Clone REA994 displays negligible binding to Fc receptors.

Alternative names

T3

Detailed product information

Technical specifications

CloneREA994
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesnon-human primate
Cross-reactivity
pigtail monkey (
Macaca nemestrina
)
,
olive baboon (
Papio anubis
)
,
african green monkey (
Chlorocebus aethiops
)
AntigenCD3
Alternative names of antigenT3
Distribution of antigenT cells, NK cells
Entrez Gene ID699582, 705270, 697814, 699467
RRIDAB_2727629, AB_2727675, AB_2727630, AB_2727676, AB_2727631, AB_2727680, AB_2727636, AB_2727681, AB_2727637, AB_2727682, AB_2727638, AB_2727677, AB_2727632, AB_2727633, AB_2727678, AB_2727634, AB_2727679, AB_2727635, AB_2727673, AB_2727628, AB_2727674

Resources for CD3 Antibody, anti-non-human primate, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

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