Clone:
REA315
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
TNFRSF17, Bcm, BCMA

Extended validation for CD269 (BCMA) Antibody, anti-human, REAfinity™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD269 (BCMA). A mixture of Human peripheral blood mononuclear cells (PBMCs) and U266 cells were stained with CD269 (BCMA) antibodies and with a suitable counterstaining. As a control, CD269 (BCMA) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD269 (BCMA). A mixture of Human peripheral blood mononuclear cells (PBMCs) and U266 cells were stained with CD269 (BCMA) antibodies and with a suitable counterstaining. As a control, CD269 (BCMA) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD269 (BCMA). A mixture of Human peripheral blood mononuclear cells (PBMCs) and U266 cells were stained with CD269 (BCMA) antibodies and with a suitable counterstaining. As a control, CD269 (BCMA) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD269 (BCMA). A mixture of Human peripheral blood mononuclear cells (PBMCs) and U266 cells were stained with CD269 (BCMA) antibodies and with a suitable counterstaining. As a control, CD269 (BCMA) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD269 (BCMA). A mixture of Human peripheral blood mononuclear cells (PBMCs) and U266 cells were stained with CD269 (BCMA) antibodies and with a suitable counterstaining. As a control, CD269 (BCMA) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD269 (BCMA) (REA315). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD269 (BCMA) (REA315). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD269 (BCMA) (REA315). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD269 (BCMA) Antibody, anti-human, REAfinity™

Overview

Clone REA315 recognizes the human CD269 antigen, a single-pass type III membrane protein which is also known as B cell maturation antigen (BCMA). CD269 is a member of the TNF-receptor superfamily that is preferentially expressed in mature B lymphocytes. It is the receptor for CD256 (TNFSF13, APRIL) and CD257 (TNFSF13B, BLyS, BAFF). CD269 promotes B cell survival and plays a role in the regulation of humoral immunity. Binding of CD269 to CD257 blocks the binding of CD257 to its receptor on the plasma membrane and inhibits CD257-triggered B lymphocyte costimulation. Overexpression of CD269 activates NF-κB and JNK, and this activation is potentiated by CD257.
Additional information: Clone REA315 displays negligible binding to Fc receptors.

Alternative names

TNFRSF17, Bcm, BCMA

Detailed product information

Technical specifications

CloneREA315
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD269 (BCMA)
Alternative names of antigenTNFRSF17, Bcm, BCMA
Molecular mass of antigen [kDa]20
Distribution of antigenB cells
Entrez Gene ID608
RRIDAB_2751592, AB_2801726, AB_2857746, AB_2857740, AB_2751638

Resources for CD269 (BCMA) Antibody, anti-human, REAfinity™

Certificates

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References for CD269 (BCMA) Antibody, anti-human, REAfinity™

Publications

  1. Yu, G. et al. (2000) APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity. Nat. Immunol. 1(3): 252-256
  2. Shu, H. B. et al. (2000) B cell maturation protein is a receptor for the tumor necrosis factor family member TALL-1. Proc. Natl. Acad. Sci. U.S.A. 97(16): 9156-9161
  3. Laabi, Y. et al. (1994) The BCMA gene, preferentially expressed during B lymphoid maturation, is bidirectionally transcribed. Nucleic Acids Res. 22(7): 1147-1154

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