Clone:
11PB6
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC
Alternative names:
KIR2DL1, KIR-K64, KIR221, NKAT, NKAT-1, p58.1, KIR2DS1

Extended validation for CD158a/h (KIR2DL1/DS1) Antibody, anti-human

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD158a/h (KIR2DL1/DS1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158a/h (KIR2DL1/DS1) antibodies and with a suitable counterstaining. As a control, CD158a/h (KIR2DL1/DS1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD158a/h (KIR2DL1/DS1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158a/h (KIR2DL1/DS1) antibodies and with a suitable counterstaining. As a control, CD158a/h (KIR2DL1/DS1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD158a/h (KIR2DL1/DS1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158a/h (KIR2DL1/DS1) antibodies and with a suitable counterstaining. As a control, CD158a/h (KIR2DL1/DS1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD158a/h (KIR2DL1/DS1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158a/h (KIR2DL1/DS1) antibodies and with a suitable counterstaining. As a control, CD158a/h (KIR2DL1/DS1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD158a/h (KIR2DL1/DS1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158a/h (KIR2DL1/DS1) antibodies and with a suitable counterstaining. As a control, CD158a/h (KIR2DL1/DS1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD158a/h (KIR2DL1/DS1) (11PB6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD158a/h (KIR2DL1/DS1) (11PB6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD158a/h (KIR2DL1/DS1) (11PB6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD158a/h (KIR2DL1/DS1) Antibody, anti-human

Overview

Clone 11PB6, also referred to as EB6, recognizes CD158a (KIR2DL1) and CD158h (KIR2DS1), two members of the killer immunoglobulin-like receptor (KIR) family expressed on CD56
dim
CD16
+
natural killer (NK) cells and CD8
+
T cells. Findings also showed reactivity of 11PB6 with KIR2DL3*005.
CD158a (KIR2DL1) is involved in the transduction of an inhibitory signal whereas CD158h (KIR2DS1) is an activating receptor. The ligands of CD158a (KIR2DL1) are HLA-Cw4 and related molecules.

Alternative names

KIR2DL1, KIR-K64, KIR221, NKAT, NKAT-1, p58.1, KIR2DS1

Detailed product information

Technical specifications

Clone11PB6
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD158a/h (KIR2DL1/DS1)
Alternative names of antigenKIR2DL1, KIR-K64, KIR221, NKAT, NKAT-1, p58.1, KIR2DS1
Molecular mass of antigen [kDa]36
Distribution of antigenNK cells
Entrez Gene ID3802
RRIDAB_2733621, AB_2857764, AB_2857752, AB_2660573, AB_871607, AB_2660575, AB_2660576, AB_871605, AB_871603, AB_2733620

Resources for CD158a/h (KIR2DL1/DS1) Antibody, anti-human

Documents and Protocols

App notes/customer reports

Cross-reactivity of KIR antibodies

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD158a/h (KIR2DL1/DS1) Antibody, anti-human

Publications

  1. Falco, M. et al. (2010) Combined genotypic and phenotypic killer cell Ig-like receptor analyses reveal KIR2DL3 alleles displaying unexpected monoclonal antibody reactivity: identification of the amino acid residues critical for staining. J. Immunol. 185: 433-441

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