In mice F4/80 is a well-established, yet not exclusive, macrophage marker. Anti-F4/80 MicroBeads UltraPure have been optimized for the rapid and simple isolation of mouse macrophages from single-cell suspensions from lymphoid and non-lymphoid tissues. The UltraPure MicroBeads greatly improve recovery and purity of the sorted population by specifically enriching viable cells.

Data and images for Anti-F4/80 MicroBeads UltraPure, mouse

Figures

Figure 1

F4/80
+
cells were isolated from spleen single-cell suspension using Anti-F4/80 MicroBeads UltraPure, two MS Columns, and an OctoMACS™ Separator. The cells were fluorescently stained with CD45-VioBlue
®
, CD11b-APC, and Anti-F4/80-FITC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD fluorescence.
Before separation
F4/80
+
cells
View details

Figure 1

F4/80
+
cells were isolated from spleen single-cell suspension using Anti-F4/80 MicroBeads UltraPure, two MS Columns, and an OctoMACS™ Separator. The cells were fluorescently stained with CD45-VioBlue
®
, CD11b-APC, and Anti-F4/80-FITC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD fluorescence.
View details

Figure 1

F4/80
+
cells were isolated from spleen single-cell suspension using Anti-F4/80 MicroBeads UltraPure, two MS Columns, and an OctoMACS™ Separator. The cells were fluorescently stained with CD45-VioBlue
®
, CD11b-APC, and Anti-F4/80-FITC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD fluorescence.

Specifications for Anti-F4/80 MicroBeads UltraPure, mouse

Overview

In mice F4/80 is a well-established, yet not exclusive, macrophage marker. Anti-F4/80 MicroBeads UltraPure have been optimized for the rapid and simple isolation of mouse macrophages from single-cell suspensions from lymphoid and non-lymphoid tissues. The UltraPure MicroBeads greatly improve recovery and purity of the sorted population by specifically enriching viable cells.

Detailed product information

F4/80 is a member of epidermal growth factor (EGF)-transmembrane 7 (TM7) family and consists of a seven-span transmembrane molecule with a large extracellular, multiple EGF module–containing, domain. F4/80 is considered as one of the most specific cell-surface markers for murine macrophages. Constitutive and high expression of F4/80 is found on most resident tissue macrophages, including spleen, microglia in the brain, Kupffer’s cells in the liver, and Langerhans cells in the skin. In addition, the expression of F4/80 can also be regulated depending on the physiological status of the cell.

Downstream applications

Macrophages isolated with Anti-F4/80 MicroBeads UltraPure can be used for phenotypical and functional analyses, e.g. cytokine secretion, phagocytosis, polarization, and tolerance induction in different experimental murine models.

Columns

MS, LS, or autoMACS
®
Columns.

Resources for Anti-F4/80 MicroBeads UltraPure, mouse

References for Anti-F4/80 MicroBeads UltraPure, mouse

Publications

  1. Wynn, T.A. et al. (2013) Macrophage biology in development, homeostasis anddisease. Nature 496: 445-455
  2. Austyn, J. M. et al. (1981) F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur. J. Immunol. 11: 805-815

Seems like you are coming from USA!
Do you want to visit our website in your country?