This application protocol describes step-by-step the immunostaining and clearing of mouse brain hemispheres using the MACS® Clearing Kit for downstream 3D imaging analysis. The MACS® Clearing Kit enables the immunostaining and clearing of up to 20 brain hemispheres from mice of any age.
2.1.1 Fixation of neonatal mouse brain hemispheres (from mice of age ≤P7)
2.1.2 Fixation of adult mouse brain hemispheres (from mice of age >P7)
2.4.1 Dehydration of mouse brain hemispheres from ≤P7 mice
2.4.2 Dehydration of mouse brain hemispheres from >P7 mice
For more information about light sheet fluorescence microscopy refer to Light sheet microscopy.
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