MBTP 33: Immunophenotyping of activated T cells within antigen-stimulated human PBMCs using flow cytometry

Miltenyi Biotec-tested panel 33 (MBTP 33)

This application protocol describes the characterization of activated T cells within antigen-stimulated peripheral blood mononuclear cells (PBMCs) via simultaneous measurement of surface markers and intracellular cytokines using flow cytometry. Prior to antibody staining PBMCs were stimulated with a cocktail of PepTivator CMV pp65, PepTivator EBV consensus, and PepTivator AdV5 Hexon from Miltenyi Biotec.

Protocol

Materials

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