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Untouched neutrophils were isolated from 8 mL of human EDTA anticoagulated whole blood using the MACSxpress ® Whole Blood Neutrophil Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD14-PerCP, CD15-PE, CD16-APC, CD45-VioBlue ®, and CD193-FITC and analyzed by flow cytometry using the MACSQuant ® Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. |
Unseparated fraction |
MACSxpress ® Whole Blood Neutrophil Isolation Kit | MACSxpress ® Whole Blood Neutrophil Isolation Kit |
After separation |
MACSxpress ® Whole Blood Neutrophil Isolation Kit | MACSxpress ® Whole Blood Neutrophil Isolation Kit |
Untouched neutrophils were isolated from 8 mL of human EDTA anticoagulated whole blood using the MACSxpress ® Whole Blood Neutrophil Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD14-PerCP, CD15-PE, CD16-APC, CD45-VioBlue ®, and CD193-FITC and analyzed by flow cytometry using the MACSQuant ® Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. |
Unseparated fraction |
MACSxpress ® Whole Blood Neutrophil Isolation Kit | MACSxpress ® Whole Blood Neutrophil Isolation Kit |
After separation |
MACSxpress ® Whole Blood Neutrophil Isolation Kit | MACSxpress ® Whole Blood Neutrophil Isolation Kit |
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