CD193 (CCR3) Antibody, anti-human

Name: CD193 (CCR3) Antibody, anti-human
Availability: InStock

CD193 (CCR3) Antibody, anti-humanExtended Validation

Clone:

5E8.4

Type of antibody:

Primary antibodies

Isotype:

mouse IgG2bκ

Applications:

FC

Alternative names:

CCR3, CC-CKR-3, CKR3, CMKBR3

Flow cytometry applications forCD193 (CCR3) Antibody, anti-human

APC
Biotin
FITC
PE
VioBlue

Conjugate:

APC

Recommended dilution:

1:11

Remark:

Cells should be stained prior to fixation, if formaldehyde is used as a fixative.

Tested:

QC tested

Products used:

130-100-618, 130-097-061

Protocols:

Cell surface flow cytometry staining protocol for CCRs (1:11)

Description:

Human peripheral blood cells after erythrocyte lysis were stained with CD193 (CCR3) antibodies or with the corresponding REA Control (S) antibodies (left images) as well as with CD16 antibodies. Flow cytometry was performed using the MACSQuant^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Extended validation for CD193 (CCR3) Antibody, anti-human

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for CD193 (CCR3). Human peripheral blood mononuclear cells (PBMCs) were stained with CD193 (CCR3) antibodies and with a suitable counterstaining. As a control, CD193 (CCR3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD193 (CCR3). Human peripheral blood mononuclear cells (PBMCs) were stained with CD193 (CCR3) antibodies and with a suitable counterstaining. As a control, CD193 (CCR3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD193 (CCR3). Human peripheral blood mononuclear cells (PBMCs) were stained with CD193 (CCR3) antibodies and with a suitable counterstaining. As a control, CD193 (CCR3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD193 (CCR3). Human peripheral blood mononuclear cells (PBMCs) were stained with CD193 (CCR3) antibodies and with a suitable counterstaining. As a control, CD193 (CCR3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Fixation data

To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using CD193 (CCR3) (5E8.4). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using CD193 (CCR3) (5E8.4). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD193 (CCR3) Antibody, anti-human

Overview

Clone 5E8.4 reacts with human CD193, a G protein–coupled receptor, also known as CCR3. CD193 binds a variety of chemokines, including eotaxin (CCL11), eotaxin-2 (CCL24), eotaxin-3 (CCL26), RANTES (CCL5), MCP-4 (CCL13), leukotactin (CCL15), and MEC (CCL28). CD193 is expressed by eosinophils, basophils, mast cells– and some T cell subsets, in particular Tʜ2 cells. CD193 regulates cell migration and activation. It is a co-receptor for HIV.

Alternative names

CCR3, CC-CKR-3, CKR3, CMKBR3

Detailed product information

Technical specifications

Clone	5E8.4
Clonality	monoclonal
Isotype	mouse IgG2bκ
Isotype control	Isotype Control Antibody, mouse IgG2b
Host	mouse
Type of antibody	Primary antibodies
Species	human
Antigen	CD193 (CCR3)
Alternative names of antigen	CCR3, CC-CKR-3, CKR3, CMKBR3
Molecular mass of antigen [kDa]	41
Distribution of antigen	dendritic cells, epithelial cells, T cells, basophils, eosinophils
Entrez Gene ID	1232
RRID	AB_2876913, AB_2655898, AB_2655899, AB_2655900, AB_2655901, AB_2655902, AB_2655903, AB_2876912

Resources for CD193 (CCR3) Antibody, anti-human

Certificates

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Applications

Extended validation