Clone:
TÜK4
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2aκ
Applications:
FC, MICS, IF, IHC, ICC
Alternative names:
LPS R

Extended validation for CD14 Antibody, anti-human

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD14. Human peripheral blood mononuclear cells (PBMCs) were stained with CD14 antibodies and with a suitable counterstaining. As a control, CD14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD14. Human peripheral blood mononuclear cells (PBMCs) were stained with CD14 antibodies and with a suitable counterstaining. As a control, CD14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD14. Human peripheral blood mononuclear cells (PBMCs) were stained with CD14 antibodies and with a suitable counterstaining. As a control, CD14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD14. Human peripheral blood mononuclear cells (PBMCs) were stained with CD14 antibodies and with a suitable counterstaining. As a control, CD14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD14. Human peripheral blood mononuclear cells (PBMCs) were stained with CD14 antibodies and with a suitable counterstaining. As a control, CD14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD14. Human peripheral blood mononuclear cells (PBMCs) were stained with CD14 antibodies and with a suitable counterstaining. As a control, CD14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD14. Human peripheral blood mononuclear cells (PBMCs) were stained with CD14 antibodies and with a suitable counterstaining. As a control, CD14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD14. Human peripheral blood mononuclear cells (PBMCs) were stained with CD14 antibodies and with a suitable counterstaining. As a control, CD14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD14 (TÜK4). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD14 (TÜK4). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD14 (TÜK4). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD14 Antibody, anti-human

Overview

Clone TÜK4 recognizes the human CD14 antigen and cross-reacts with non-human primate CD14. The CD14 antigen is a high affinity receptor for lipopolysaccharides (LPS) and LPS–binding protein (LBP)–complexes. It is part of the functional heteromeric LPS receptor complex comprised of CD14, TLR4, and MD-2.
CD14 is strongly expressed on most human monocytes and macrophages in peripheral blood, other body fluids, and various tissues, such as lymph nodes and spleen. CD14 is expressed at high levels, also on a few CD1c (BDCA-1)
+
CD2
+
myeloid dendritic cells and at low levels on neutrophilic granulocytes.
Ex vivo
differentiation of monocytes to dendritic cells is associated with down–regulation of CD14 antigen expression.

Alternative names

LPS R

Detailed product information

Technical specifications

CloneTÜK4
Clonalitymonoclonal
Isotypemouse IgG2aκ
Isotype controlIsotype Control Antibody, mouse IgG2a
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate, other
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
,
pigtail monkey (
Macaca nemestrina
)
,
cotton-top tamarin (
Saguinus oedipus
)
, pig, cow, sheep, goat, dog, mink, rabbit
AntigenCD14
Alternative names of antigenLPS R
Molecular mass of antigen [kDa]37
Distribution of antigenB cells, dendritic cells, epithelial cells, granulocytes, Langerhans cells, macrophages, monocytes, osteoclasts, platelets, plasma cells, liver
Entrez Gene ID929
RRIDAB_2725974, AB_2726250, AB_2725975, AB_2726246, AB_2725971, AB_2726255, AB_2725980, AB_2726256, AB_2725981, AB_2726253, AB_2725978, AB_2726251, AB_2725976, AB_2726252, AB_2725977, AB_2726247, AB_2725972, AB_2726254, AB_2725979, AB_2726248, AB_2725973, AB_2726249

Resources for CD14 Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

Reviews for CD14 Antibody, anti-human

Staining Peripheral Blood with Anti-CD14 APC

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CD14-APC, human (130-113-143)

Will continue to use this antibody.

Staining Peripheral Blood with Anti-CD14 APC

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CD14-APC, human (130-113-705)

Will continue to use this antibody.

References for CD14 Antibody, anti-human

Publications

  1. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
  2. Meissner, F. et al. (2010) Inflammasome activation in NADPH oxidase defective mononuclear phagocytes from patients with chronic granulomatous disease. Blood 116(9): 1570-1573
  3. Wilson, A. D. et al. (1995)
    Selection of monoclonal antibodies for the identification of lymphocyte surface antigens in the New World primate
    Saguinus oedipus oedipus
    (cotton top tamarin).
    J. Immunol. Methods 178: 195-200
  4. Neu, C. et al. (2013) CD14-dependent monocyte isolation enhances phagocytosis of listeria monocytogenes by proinflammatory, GM-CSF-derived macrophages. PLoS One 8(6): e66898
  5. Paccou, J. et al. (2013)
    Determination and modulation of total and surface calcium-sensing receptor expression in monocytes
    in vivo
    and
    in vitro
    .
    PLoS One 8(10): e74800
  6. Jacobsen, C. N. et al. (1993) Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species. Vet. Immunol. Immunopathol. 39: 461-466
  7. Goyer, S. M. and Ferrero, E. (1987) Biochemical analysis of myeloid antigen and cDNA expression of gp55 (CD14). Oxford, Oxford University Press

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