Clone:
7A3-3
Type of antibody:
Primary antibodies, Functional-grade antibodies
Isotype:
mouse IgG1, mouse IgG1κ
Applications:
MC, ICFC, FA
Alternative names:
BCGF-1, BCGF1, BSF-1, BSF1

Extended validation for IL-4 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with 7A3-3
8D4-8++
MP4-25D2-
3010.211-
3007++
REA895-
1A6.10-
Cells were incubated with an excess of purified unconjugated IL-4 (7A3-3) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for IL-4 Antibody, anti-human

Overview

Interleukin-4 (IL-4) is predominantly secreted by activated CD4
+
memory and effector Tʜ2 cells, basophils, and mast cells. It induces and supports humoral immune responses for the neutralization of extracellular pathogens. The Tʜ2 types of immune mechanisms can also be involved in immunological disorders such as IgE-mediated allergy.
The Anti-IL‑4 antibody has been designed for intracellular staining of IL‑4–producing cells. Cells can be stimulated for IL‑4–production, e.g., by polyclonal stimulation with mitogens. For induction of IL‑4 production by antigen-specific T cells, cells are restimulated with respective antigen. IL‑4 can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IL‑4–producing cells can be stained intracellularly with the Anti-IL‑4 antibody. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IL‑4–producing cells. Magnetically enriched cells can be stained intracellularly for IL‑4 production directly on the MACS
®
Column. This procedure ensures higher sensitivity of detection and minimizes loss of cells during washing procedures for cytokine analysis of rare cells, e.g., CD4
+
T cells in HIV patients, or other cell sources than peripheral blood mononuclear cells (PBMCs), e.g., bronchoalveolar lavages, or synovial fluids.

Alternative names

BCGF-1, BCGF1, BSF-1, BSF1

Detailed product information

Technical specifications

Clone7A3-3
Clonalitymonoclonal
Isotypemouse IgG1κ, mouse IgG1
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies, Functional-grade antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
AntigenIL-4
Alternative names of antigenBCGF-1, BCGF1, BSF-1, BSF1
Molecular mass of antigen [kDa]15
Entrez Gene ID3565
RRIDAB_10831210, AB_2661074, AB_2661075, AB_2661076

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