Clone:
VIT4
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2aκ
Applications:
FC, MICS, IF, IHC, ICC
Alternative names:
CD4mut, L3T4, Leu-3, T4

Extended validation for CD4 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with VIT4
RPA-T4++
REA623-
M-T466-
M-T321-
SK3-
OKT4-
Cells were incubated with an excess of purified unconjugated CD4 (VIT4) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD4. Human peripheral blood mononuclear cells (PBMCs) were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Monocytes were excluded from the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD4. Human peripheral blood mononuclear cells (PBMCs) were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Monocytes were excluded from the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD4. Human peripheral blood mononuclear cells (PBMCs) were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Monocytes were excluded from the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD4. Human peripheral blood mononuclear cells (PBMCs) were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Monocytes were excluded from the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD4. Human peripheral blood mononuclear cells (PBMCs) were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Monocytes were excluded from the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD4. Human peripheral blood mononuclear cells (PBMCs) were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Monocytes were excluded from the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD4. Human peripheral blood mononuclear cells (PBMCs) were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Monocytes were excluded from the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD4. Human peripheral blood mononuclear cells (PBMCs) were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Monocytes were excluded from the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD4. Human peripheral blood mononuclear cells (PBMCs) were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Monocytes were excluded from the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD4. Human peripheral blood mononuclear cells (PBMCs) were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Monocytes were excluded from the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD4. Human peripheral blood mononuclear cells (PBMCs) were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Monocytes were excluded from the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD4. Human peripheral blood mononuclear cells (PBMCs) were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Monocytes were excluded from the analysis. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD4 (VIT4). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD4 (VIT4). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD4 (VIT4). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD4 Antibody, anti-human

Overview

Clone VIT4 recognizes the human CD4 antigen which is highly expressed on human T helper cells and thymocytes, and at lower levels on monocytes and dendritic cells. It is responsible for the recognition of the MHC class II antigen.
VIT4 can be used for evaluating the transfection efficiency of cells transfected with pMACS 4.1 or pMACS 4-IRES when using the MACSelect™ – 4 Transfected Cell Selection Kit.

Alternative names

CD4mut, L3T4, Leu-3, T4

Detailed product information

Technical specifications

CloneVIT4
Clonalitymonoclonal
Isotypemouse IgG2aκ
Isotype controlIsotype Control Antibody, mouse IgG2a
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD4
Alternative names of antigenCD4mut, L3T4, Leu-3, T4
Molecular mass of antigen [kDa]48
Distribution of antigendendritic cells, granulocytes, Langerhans cells, lymphocytes, macrophages, monocytes, T cells, neutrophils, T helper cells, thymocytes
Entrez Gene ID920
RRIDAB_2726024, AB_2726702, AB_2726306, AB_2726031, AB_2726300, AB_2726025, AB_2726296, AB_2726021, AB_2726305, AB_2726030, AB_2726307, AB_2726032, AB_2726303, AB_2726028, AB_2726301, AB_2726026, AB_2726302, AB_2726027, AB_2726297, AB_2726022, AB_2726304, AB_2726029, AB_2726298, AB_2726023, AB_2751058, AB_2751052, AB_2726299

Reviews for CD4 Antibody, anti-human

Staining Peripheral Blood with Anti-CD4 VioGreen

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CD4-VioGreen, human (130-113-221)

Works well. Reproducible results.

Staining Peripheral Blood with Anti-CD4 VioGreen

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CD4-VioGreen, human (130-113-783)

Works well. Reproducible results.

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