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Several of our in vitro experiments require purified primary CD4+ T cells . We have worked with total PBMC in the past, but found that using purified CD4+ T cells gave more consistent results. After comparing different cell separation technologies, we settled upon CD4+ Microbeads from Miltenyi given their consistency, quick protocol, and purity of the isolated cells.
We use these beads to purify CD4+ T cells from human PBMCs. The protocol is very straight forward and gives consistent results.
Our lab studies HIV immunology which requires the isolation of CD4+ cells for in vitro infection. These beads provide a reliable way to isolate this cell population from human PBMCs for downstream applications.
Several of our in vitro experiments require purified primary CD4+ T cells . We have worked with total PBMC in the past, but found that using purified CD4+ T cells gave more consistent results. After comparing different cell separation technologies, we settled upon CD4+ Microbeads from Miltenyi given their consistency, quick protocol, and purity of the isolated cells.
We use these beads to purify CD4+ T cells from human PBMCs. The protocol is very straight forward and gives consistent results.
Our lab studies HIV immunology which requires the isolation of CD4+ cells for in vitro infection. These beads provide a reliable way to isolate this cell population from human PBMCs for downstream applications.
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