CD45 (TIL) MicroBeads, mouse have been optimized for the isolation of tumor-infiltrating leukocytes from mouse tumors.

Data and images for CD45 (TIL) MicroBeads, mouse

Figures

Figure 1

A tumor induced by the B16F10 cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD45
+
TILs were isolated from the resulting single-cell suspension using CD45 (TIL) MicroBeads, mouse, an MS Column, and a MiniMACS™ Separator.
Cells were fluorescently stained with CD45-PE and Labeling Check Reagent-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
CD45
+
cells
View details

Figure 1

A tumor induced by the B16F10 cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD45
+
TILs were isolated from the resulting single-cell suspension using CD45 (TIL) MicroBeads, mouse, an MS Column, and a MiniMACS™ Separator.
Cells were fluorescently stained with CD45-PE and Labeling Check Reagent-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

A tumor induced by the B16F10 cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD45
+
TILs were isolated from the resulting single-cell suspension using CD45 (TIL) MicroBeads, mouse, an MS Column, and a MiniMACS™ Separator.
Cells were fluorescently stained with CD45-PE and Labeling Check Reagent-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD45 (TIL) MicroBeads, mouse

Overview

CD45 (TIL) MicroBeads, mouse have been optimized for the isolation of tumor-infiltrating leukocytes from mouse tumors.

Detailed product information

Background information

CD45 (TIL) MicroBeads, mouse enable a positive selection of CD45
+
leukocytes from solid mouse tumors, e.g., CT26.WT, 4T1, and B16F10 and have specifically been developed for an optimal isolation of tumor-infiltrating leukocytes (TILs) from mouse tumors. The CD45 antigen is expressed on all cells of hematopoietic origin except erythrocytes and platelets.

Columns

MS, LS, autoMACS
®
Columns, or Multi-24 Column Blocks

Resources for CD45 (TIL) MicroBeads, mouse

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CD45 (TIL) MicroBeads, mouse

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