CD4/CD8 (TIL) MicroBeads, mouse

CD4/CD8 (TIL) MicroBeads, mouse have been optimized for the isolation of CD4
+
and CD8
+
tumor-infiltrating lymphocytes from mouse tumors.

Data and images for CD4/CD8 (TIL) MicroBeads, mouse

Figures

Figure 1

A tumor induced by the B16-OVA cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD4
+
and CD8
+
TILs were isolated from the single-cell suspension using CD4/8 (TIL) MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD4-PE-Vio
®
615 and CD8b-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and Viobility™ 405/520 Fixable Dye fluorescence.
Before separation
CD4
+
and CD8
+
cells
View details

Figure 1

A tumor induced by the B16-OVA cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD4
+
and CD8
+
TILs were isolated from the single-cell suspension using CD4/8 (TIL) MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD4-PE-Vio
®
615 and CD8b-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and Viobility™ 405/520 Fixable Dye fluorescence.
View details

Figure 1

A tumor induced by the B16-OVA cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD4
+
and CD8
+
TILs were isolated from the single-cell suspension using CD4/8 (TIL) MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD4-PE-Vio
®
615 and CD8b-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and Viobility™ 405/520 Fixable Dye fluorescence.

Specifications for CD4/CD8 (TIL) MicroBeads, mouse

Overview

CD4/CD8 (TIL) MicroBeads, mouse have been optimized for the isolation of CD4
+
and CD8
+
tumor-infiltrating lymphocytes from mouse tumors.

Detailed product information

Background information

CD4/CD8 (TIL) MicroBeads, mouse have been developed for the isolation of conventional T cells from single-cell suspensions of mouse tumors. The CD4 antigen is expressed on T helper cells, regulatory T cells, and at lower levels on subpopulations of NKT cells and dendritic cells. The CD8b antigen is expressed on cytotoxic T cells.

Columns

MS, LS, autoMACS
®
Columns, or Multi-24 Column Blocks

Resources for CD4/CD8 (TIL) MicroBeads, mouse

Documents and Protocols

Scientific posters

Optimized workflow improves the characterization of tumor-infiltrating T cells

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CD4/CD8 (TIL) MicroBeads, mouse

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