CD44 MicroBeads have been specifically designed and optimized for the isolation or depletion of CD44
+
cells from single-cell suspensions from solid tumors, including breast, pancreatic, colon, and prostate tissues or cancer cell lines.

Data and images for CD44 MicroBeads, human

Figures

Figure 1

CD44
+
cells were isolated from a mixture of U937 (CD44
+
) and 1881 (CD44
) cell lines. CD44
+
cells were isolated using the CD44 MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD44-PE and analyzed by flow cytometry using the MACSQuant® Analyzer.
Unseparated fraction
CD44
cells
View details

Figure 1

CD44
+
cells were isolated from a mixture of U937 (CD44
+
) and 1881 (CD44
) cell lines. CD44
+
cells were isolated using the CD44 MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD44-PE and analyzed by flow cytometry using the MACSQuant® Analyzer.
View details

Figure 1

CD44
+
cells were isolated from a mixture of U937 (CD44
+
) and 1881 (CD44
) cell lines. CD44
+
cells were isolated using the CD44 MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD44-PE and analyzed by flow cytometry using the MACSQuant® Analyzer.
CD44
+
cells
View details

Figure 1

CD44
+
cells were isolated from a mixture of U937 (CD44
+
) and 1881 (CD44
) cell lines. CD44
+
cells were isolated using the CD44 MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD44-PE and analyzed by flow cytometry using the MACSQuant® Analyzer.

Specifications for CD44 MicroBeads, human

Overview

CD44 MicroBeads have been specifically designed and optimized for the isolation or depletion of CD44
+
cells from single-cell suspensions from solid tumors, including breast, pancreatic, colon, and prostate tissues or cancer cell lines.

Detailed product information

Background information

CD44 is a marker for many types of cancer stem cells (CSC), including breast CSCs that possess higher tumorigenicity and metastatic potential
1
, colorectal
2
, pancreatic
3
, and prostate
4,5
CSCs. In addition, expression was observed in several cancers as well as on carcinoma cell lines. Here, it plays a role in cancer cell migration and matrix adhesion in response to a cellular microenvironment, thus enhancing cellular aggregation and tumor cell growth
6
. CD44 is also expressed on mesodermal cells, such as hematopoietic, fibroblastic, and glial cells.

Columns

For positive selection: LS, XS, or autoMACS
®
Columns. For depletion: LD, CS, D, or autoMACS Columns.
Note: This product is not suitable for cell separation with an MS Column.

References for CD44 MicroBeads, human

Publications

  1. Al-Hajj, M. et al. (2003) Prospective identification of tumorigenic breast cancer cells. Proc. Natl. Acad. Sci. U.S.A. 100: 3983-3988
  2. Dalerba, P. et al. (2007) Phenotypic characterization of human colorectal cancer stem cells. Proc. Natl. Acad. Sci. U.S.A. 104: 10158-10163
  3. Li, C. P. et al. (2007) Identification of pancreatic cancer stem cells. Cancer Res. 67: 1030-1037
  4. Patrawala, L. et al. (2006)
    Highly purified CD44
    +
    prostate cancer cells from xenograft human tumors are enriched in tumorigenic and metastatic progenitor cells.
    Oncogene 25: 1696-1708
  5. Collins, A. T. et al. (2005) Prospective identification of tumorigenic prostate cancer stem cells. Cancer Res. 65: 10946-10951
  6. Aruffo, A. et al. (1990) CD44 is the principal cell surface receptor for hyaluronate. Cell 61: 1303-1313

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