The CD24 MicroBead Kit has been developed and optimized for the depletion of CD24-expressing cells from cancer cell lines or single-cell suspensions created from solid tissues. It can also be used for positive selection of cancer stem cells from cancer cell lines or single-cell suspensions created from solid tissues.

Data and images for CD24 MicroBead Kit, human

Figures

Figure 1

CD24
+
cells were depleted from the CML cell line K562 using the CD24 MicroBead Kit, an LD Column, and a QuadroMACS™ Separator. Cells were fluorescently labeled with CD24-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
A:
B:
Unseparated fraction
CD24
-
cell fraction
View details

Figure 1

CD24
+
cells were depleted from the CML cell line K562 using the CD24 MicroBead Kit, an LD Column, and a QuadroMACS™ Separator. Cells were fluorescently labeled with CD24-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

CD24
+
cells were depleted from the CML cell line K562 using the CD24 MicroBead Kit, an LD Column, and a QuadroMACS™ Separator. Cells were fluorescently labeled with CD24-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD24 MicroBead Kit, human

Overview

The CD24 MicroBead Kit has been developed and optimized for the depletion of CD24-expressing cells from cancer cell lines or single-cell suspensions created from solid tissues. It can also be used for positive selection of cancer stem cells from cancer cell lines or single-cell suspensions created from solid tissues.

Detailed product information

Columns

For depletion: LD Columns for optimal depletion efficiency; MS or LS Columns for cells which strongly express the CD24 antigen; autoMACS
®
Columns.
For positive selection: MS, LS, or autoMACS Columns.

References for CD24 MicroBead Kit, human

Publications

  1. Al-Hajj, M. et al. (2003) Prospective identification of tumorigenic breast cancer cells. Proc. Natl. Acad. Sci. U.S.A. 100: 3983-3988
  2. Gao, M. Q. et al. (2010)
    CD24
    +
    cells from hierarchically organized ovarian cancer are enriched in cancer stem cells.
    Oncogene 29: 2672-2680
  3. Li, C. P. et al. (2007) Identification of pancreatic cancer stem cells. Cancer Res. 67: 1030-1037

Related products for
CD24 MicroBead Kit, human

11 products available | view all

Seems like you are coming from USA!
Do you want to visit our website in your country?