CD326 (EpCAM) MicroBeads, human

CD326 (EpCAM) MicroBeads, human

CD326 (EpCAM) MicroBeads can be used for the:

positive selection or depletion of epithelial cells or epithelial tumor cells from single-cell preparations of tissues and organs,
enrichment of circulating or disseminated epithelial tumor cells expressing CD326 from peripheral blood, bone marrow, or even lymph nodes, serous effusions, or stool samples¹ of patients with carcinomas,
positive selection of functional pluripotent hepatic progenitor cells (hepatic stem cells and hepatoblasts) from fetal, neonatal, pediatric, and adult liver

Product data and images for CD326 (EpCAM) MicroBeads, human

Figures

Figure 1

Enrichment of tumor cells from PBMCs spiked with cells of the breast cancer cell line BT474. Separation was performed using an MS Column and CD326 (EpCAM) MicroBeads.

Cell mixture before separation (6×10 ⁷ PBMCs were mixed with 50,000 tumor cells)	Positive fraction with enriched breast cancer cells (1.54×10 ⁵ total cells)
View details CD326 (EpCAM) MicroBeads, human Figure 1 Enrichment of tumor cells from PBMCs spiked with cells of the breast cancer cell line BT474. Separation was performed using an MS Column and CD326 (EpCAM) MicroBeads.	View details CD326 (EpCAM) MicroBeads, human Figure 1 Enrichment of tumor cells from PBMCs spiked with cells of the breast cancer cell line BT474. Separation was performed using an MS Column and CD326 (EpCAM) MicroBeads.

Figure 2

CD326 (EpCAM)
⁺
tumor cells isolated from pleural effusion of a patient with adeno-carcinoma using CD326 (EpCAM) MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were stained with CD326 (EpCAM)-FITC and Anti-FITC Alkaline Phosphatase plus substrate.
(Courtesy of Dr. Motherby and Prof. Böcking, Inst. of Cytopathol., Univ. Düsseldorf, Germany.)

Figure 2

CD326 (EpCAM)

⁺

tumor cells isolated from pleural effusion of a patient with adeno-carcinoma using CD326 (EpCAM) MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were stained with CD326 (EpCAM)-FITC and Anti-FITC Alkaline Phosphatase plus substrate.

(Courtesy of Dr. Motherby and Prof. Böcking, Inst. of Cytopathol., Univ. Düsseldorf, Germany.)

Specifications for CD326 (EpCAM) MicroBeads, human

Overview

CD326 (EpCAM) MicroBeads can be used for the:

positive selection or depletion of epithelial cells or epithelial tumor cells from single-cell preparations of tissues and organs,
enrichment of circulating or disseminated epithelial tumor cells expressing CD326 from peripheral blood, bone marrow, or even lymph nodes, serous effusions, or stool samples¹ of patients with carcinomas,
positive selection of functional pluripotent hepatic progenitor cells (hepatic stem cells and hepatoblasts) from fetal, neonatal, pediatric, and adult liver tissue^2,3,
positive selection of tumor exosomes from peripheral blood⁴,
enrichment of exfoliated epithelial cells from gastric aspirates and stool samples of preterm infants⁵,
purification of primary tumor cell cultures⁶.

Detailed product information

Background information

CD326, also known as human epithelial antigen (HEA), epithelial cell adhesion molecule (EpCAM), or epithelial-specific antigen (ESA) expression is seen in the majority of carcinomas. It is expressed on cells of epithelial origin, epithelium-derived tumor cells

⁷

, circulating tumor cells, and cancer stem cells.

Downstream applications

Tumor cells enriched by CD326 (EpCAM) MicroBeads can be cultured

⁸

and analyzed by immunocytochemistry

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, flow cytometry, or molecular biology techniques, for example, RT-PCR

¹⁰

.

Columns

MS, LS, XS, or autoMACS

^®

Columns.

Resources for CD326 (EpCAM) MicroBeads, human

Documents and Protocols

Brochures/posters

Cancer Research - Understanding cancer begins with the cell

App notes/customer reports

High-sensitivity detection and analysis of rare cells by integrated magnetic enrichment and flow cytometry using the MACSQuant® Analyzer.

References for CD326 (EpCAM) MicroBeads, human

Publications

Molloy, T. J. et al. (2008) Towards an optimized platform for the detection, enrichment, and semi-quantitation circulating tumor cells. Breast Cancer Res. Treat. 112: 297-307
Lewin, M. H. et al. (2006) Red meat enhances the colonic formation of the DNA adduct O6-carboxymethyl guanine: implications for colorectal cancer risk. Cancer Res. 66: 1859-1865
Schmelzer, E. et al. (2006) The phenotypes of pluripotent human hepatic progenitors. Stem Cells 24: 1852-1858
Schmelzer, E. et al. (2007) Human hepatic stem cells from fetal and postnatal donors. J. Exp. Med. 204: 1973-1987
Taylor, D. D. and Gercel-Taylor, C. (2008) MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer. Gynecol. Oncol. 110: 13-21
Kaeffer, B. et al. (2007) Recovery of exfoliated cells from the gastrointestinal tract of premature infants: a new tool to perform "noninvasive biopsies?". Pediatr. Res. 62: 564-569
Moldenhauer, G. et al. (1987) Epithelium specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker. Br. J. Cancer 56: 714-721
Leinung, S. et al. (2000) Cytokeratin‑positive cells in bone marrow in comparison with other prognostic factors in colon carcinoma. Langenbecks Arch. Surg. 385: 337-343
Krüger, W. et al. (2000) Immunomagnetic tumor cell selection--implications for the detection of disseminated cancer cells. Transfusion 40: 1489-1493

Data and images