The CD294 (CRTH2) MicroBead Kit was developed for the positive selection or depletion of CD294
+
cells from human PBMCs.

Data and images for CD294 (CRTH2) MicroBead Kit, human

Figures

Figure 1

Isolation of human peripheral blood CD4
+
CD294
+
cells using the CD4
+
T Cell Isolation Kit II and the CD294 (CRTH2) MicroBead Kit, an LD Column in a MidiMACS™ Separator, and two MS Columns in a MiniMACS™ Separator.
PBMCs before separation
Pre-enriched CD4
+
T cells
View details

Figure 1

Isolation of human peripheral blood CD4
+
CD294
+
cells using the CD4
+
T Cell Isolation Kit II and the CD294 (CRTH2) MicroBead Kit, an LD Column in a MidiMACS™ Separator, and two MS Columns in a MiniMACS™ Separator.
View details

Figure 1

Isolation of human peripheral blood CD4
+
CD294
+
cells using the CD4
+
T Cell Isolation Kit II and the CD294 (CRTH2) MicroBead Kit, an LD Column in a MidiMACS™ Separator, and two MS Columns in a MiniMACS™ Separator.
Isolated CD4
+
CD294
+
T cells
View details

Figure 1

Isolation of human peripheral blood CD4
+
CD294
+
cells using the CD4
+
T Cell Isolation Kit II and the CD294 (CRTH2) MicroBead Kit, an LD Column in a MidiMACS™ Separator, and two MS Columns in a MiniMACS™ Separator.

Specifications for CD294 (CRTH2) MicroBead Kit, human

Overview

The CD294 (CRTH2) MicroBead Kit was developed for the positive selection or depletion of CD294
+
cells from human PBMCs.

Detailed product information

Background information

CD294, also known as CRTH2, is a receptor for prostaglandin D
2
. CD294 is expressed on T helper type 2 (T
H
2) cells, a subpopulation of CD4
+
T cells, but is not present on T
H
1 cells. CD294 is highly expressed on peripheral blood basophils and eosinophils. Furthermore, it is present on a small population of CD8
+
T cells as well as on a subpopulation of dendritic cells.
For isolation of the CD294
+
T helper cell subset, the CD294 (CRTH2) MicroBead Kit can be combined with the CD4
+
T Cell Isolation Kit or the CD4 MultiSort Kit. A special protocol is available.

Detailed separation procedure

The CD294
+
cells are fluorescently and indirectly magnetically labeled with CD294-PE and Anti-PE MicroBeads.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

Resources for CD294 (CRTH2) MicroBead Kit, human

References for CD294 (CRTH2) MicroBead Kit, human

Publications

  1. Cosmi, L. et al. (2000) CRTH2 is the most reliable marker for the detection of circulating human type 2 Tʜ and type 2 T cytotoxic cells in health and disease. Eur. J. Immunol. 30: 2972-2979

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