CD123 MicroBeads have been developed for the concurrent isolation of human basophilic granulocytes and plasmacytoid dendritic cells.

Data and images for CD123 MicroBeads, human

Figures

Figure 1

CD123
+
basophils and plasmacytoid dendritic cells were isolated from human PBMCs using CD123 MicroBeads and two MS Columns. Cells were fluorescently stained with CD123-PE, CD203c-APC, and CD303 (BDCA-2)-FITC, and analyzed by flow cytometry.
Unseparated fraction
View details

Figure 1

CD123
+
basophils and plasmacytoid dendritic cells were isolated from human PBMCs using CD123 MicroBeads and two MS Columns. Cells were fluorescently stained with CD123-PE, CD203c-APC, and CD303 (BDCA-2)-FITC, and analyzed by flow cytometry.
View details

Figure 1

CD123
+
basophils and plasmacytoid dendritic cells were isolated from human PBMCs using CD123 MicroBeads and two MS Columns. Cells were fluorescently stained with CD123-PE, CD203c-APC, and CD303 (BDCA-2)-FITC, and analyzed by flow cytometry.
After separation
View details

Figure 1

CD123
+
basophils and plasmacytoid dendritic cells were isolated from human PBMCs using CD123 MicroBeads and two MS Columns. Cells were fluorescently stained with CD123-PE, CD203c-APC, and CD303 (BDCA-2)-FITC, and analyzed by flow cytometry.
View details

Figure 1

CD123
+
basophils and plasmacytoid dendritic cells were isolated from human PBMCs using CD123 MicroBeads and two MS Columns. Cells were fluorescently stained with CD123-PE, CD203c-APC, and CD303 (BDCA-2)-FITC, and analyzed by flow cytometry.

Specifications for CD123 MicroBeads, human

Overview

CD123 MicroBeads have been developed for the concurrent isolation of human basophilic granulocytes and plasmacytoid dendritic cells.

Detailed product information

Background information

In peripheral blood, the CD123 antigen is expressed at high levels only on plasmacytoid dendritic cells and basophilic granulocytes. By contrast, monocytes, eosinophilic granulocytes, myeloid dendritic cells, and subsets of hematopoietic progenitor cells express CD123 only at low levels.

Applications

Positive selection of human basophilic granulocytes and plasmacytoid dendritic cells.

Columns

MS, LS, or autoMACS
®
Columns.

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CD123 MicroBeads, human

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