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Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c + cells or CD123 brightCD303 – cells because CD123 is expressed at high levels on both basophils and CD303 + plasmacytoid dendritic cells. |
Unseparated fraction | |
A: | B: |
Basophil Isolation Kit II, humanFigure 1Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c + cells or CD123 brightCD303 – cells because CD123 is expressed at high levels on both basophils and CD303 + plasmacytoid dendritic cells. | Basophil Isolation Kit II, humanFigure 1Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c + cells or CD123 brightCD303 – cells because CD123 is expressed at high levels on both basophils and CD303 + plasmacytoid dendritic cells. |
Isolated basophils | |
A: | B: |
Basophil Isolation Kit II, humanFigure 1Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c + cells or CD123 brightCD303 – cells because CD123 is expressed at high levels on both basophils and CD303 + plasmacytoid dendritic cells. | Basophil Isolation Kit II, humanFigure 1Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c + cells or CD123 brightCD303 – cells because CD123 is expressed at high levels on both basophils and CD303 + plasmacytoid dendritic cells. |
Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c + cells or CD123 brightCD303 – cells because CD123 is expressed at high levels on both basophils and CD303 + plasmacytoid dendritic cells. |
Unseparated fraction | |
A: | B: |
Basophil Isolation Kit II, humanFigure 1Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c + cells or CD123 brightCD303 – cells because CD123 is expressed at high levels on both basophils and CD303 + plasmacytoid dendritic cells. | Basophil Isolation Kit II, humanFigure 1Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c + cells or CD123 brightCD303 – cells because CD123 is expressed at high levels on both basophils and CD303 + plasmacytoid dendritic cells. |
Isolated basophils | |
A: | B: |
Basophil Isolation Kit II, humanFigure 1Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c + cells or CD123 brightCD303 – cells because CD123 is expressed at high levels on both basophils and CD303 + plasmacytoid dendritic cells. | Basophil Isolation Kit II, humanFigure 1Basophils were enriched from human PBMCs using the Basophil Isolation Kit II, a MidiMACS™ Separator and an LS Column. Cells were stained with CD203c-APC, CD123-PE and CD303 (BDCA-2)-FITC. Basophils were detected as CD203c + cells or CD123 brightCD303 – cells because CD123 is expressed at high levels on both basophils and CD303 + plasmacytoid dendritic cells. |
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