Clone:
FR3-16A11
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC
Alternative names:
ENPP3, B10, NPP3, PD-IBETA, PDNP3, E-NPP3, PD-1b

Extended validation for CD203c Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with FR3-16A11
REA826++
NP4D6++
Cells were incubated with an excess of purified unconjugated CD203c (FR3-16A11) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD203c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD203c antibodies and with a suitable counterstaining. As a control, CD203c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD203c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD203c antibodies and with a suitable counterstaining. As a control, CD203c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD203c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD203c antibodies and with a suitable counterstaining. As a control, CD203c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD203c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD203c antibodies and with a suitable counterstaining. As a control, CD203c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD203c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD203c antibodies and with a suitable counterstaining. As a control, CD203c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD203c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD203c antibodies and with a suitable counterstaining. As a control, CD203c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD203c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD203c antibodies and with a suitable counterstaining. As a control, CD203c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD203c (FR3-16A11). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD203c (FR3-16A11). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD203c (FR3-16A11). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD203c Antibody, anti-human

Overview

Clone FR3-16A11 recognizes CD203c, a glycosylated type II transmembrane molecule that belongs to the family of ecto-nucleotide pyrophosphatase/ phosphodiesterase (E-NPP3) enzymes. Among hematopoietic cells, expression of CD203c is restricted to basophils as well as to mast cells and their precursors, and has been described as specific for this lineage. Protein and/or mRNA expression of CD203c has also been found in solid tissues such as uterus or prostate. Basophils and mast cells are key producers of mediators that drive the onset of inflammatory responses, e.g., in allergy. Allergen challenge leads to a rapid up-regulation of activation markers such as CD203c or CD63. Due to its restricted expression pattern, CD203c is discussed as a specific marker to monitor the allergen-induced activation of basophils, e.g., in flow cytometric basophil activation tests of the peripheral blood.

Alternative names

ENPP3, B10, NPP3, PD-IBETA, PDNP3, E-NPP3, PD-1b

Detailed product information

Technical specifications

CloneFR3-16A11
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD203c
Alternative names of antigenENPP3, B10, NPP3, PD-IBETA, PDNP3, E-NPP3, PD-1b
Molecular mass of antigen [kDa]100
Distribution of antigenmast cells, basophils, liver, pancreas, small intestine
Entrez Gene ID5169
RRIDAB_2811555, AB_615065, AB_2660234, AB_2660235, AB_615067, AB_2904811, AB_2904810, AB_2811581

Resources for CD203c Antibody, anti-human

Certificates

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References for CD203c Antibody, anti-human

Publications

  1. Bühring, H. J. et al. (1999) The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multipotent and unipotent progenitors. Blood 94: 2343-2356
  2. Goding, J. W. (2000) Ecto-enzymes: physiology meets pathology. J. Leukoc. Biol. 67: 285-311
  3. Kahlert, H. et al. (2003) Measurement of basophil-activating capacity of grass pollen allergens, allergoids Clin. Exp. Allergy 33: 1266-1272
  4. Bühring, H. J. et al. (2004) The basophil-specific ectoenzyme E-NPP3 (CD203c) as a marker for cell activation and allergy diagnosis. Int. Arch. Allergy Immunol. 133: 317-329
  5. Hauswirth, A. W. et al. (2002) Recombinant allergens promote expression of CD203c on basophils in sensitized individuals. J. Allergy Clin. Immunol. 110: 102-109

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