AstroMACS Separation Buffer is a PBS-based buffer optimized for dead cell removal during adult astrocyte isolation. We highly recommend using AstroMACS Separation Buffer for optimal results of adult astrocyte cultivation.

Data and images for AstroMACS Separation Buffer

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Figure 1

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AstroMACS Separation Buffer removes dead cells and enhances adult astrocyte culture:
Adult astrocytes were isolated from the brain of 8-week-old mice using the Adult Brain Dissociation Kit and the Anti-ACSA-2 MicroBead Kit in combination with (A) or without (B) the AstroMACS Separation Buffer.
Cells were then cultivated in AstroMACS Medium at a density of 100,000 cells /well in a 24-well Imaging Plate for 7 days. Cells were fixed and stained with Anti-GLAST antibody (green) and DAPI (blue).

Figure 1

AstroMACS Separation Buffer removes dead cells and enhances adult astrocyte culture:
Adult astrocytes were isolated from the brain of 8-week-old mice using the Adult Brain Dissociation Kit and the Anti-ACSA-2 MicroBead Kit in combination with (A) or without (B) the AstroMACS Separation Buffer.
Cells were then cultivated in AstroMACS Medium at a density of 100,000 cells /well in a 24-well Imaging Plate for 7 days. Cells were fixed and stained with Anti-GLAST antibody (green) and DAPI (blue).

Specifications for AstroMACS Separation Buffer

Overview

AstroMACS Separation Buffer is a PBS-based buffer optimized for dead cell removal during adult astrocyte isolation. We highly recommend using AstroMACS Separation Buffer for optimal results of adult astrocyte cultivation.

Detailed product information

Background information

Adult neural cells are known to be fragile, and adult brain dissociation often generates numerous dead cells, which affect the astrocyte viability in culture. AstroMACS Separation Buffer effectively removes the dead cells during cell separation; in combination with AstroMACS Medium, they together lead to healthy adult astrocytes in culture.

Applications

Cultivation of healthy adult astrocytes, together with AstroMACS Medium.

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