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AstroMACS Medium

Name: AstroMACS Medium
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AstroMACS Medium

AstroMACS Medium is an optimized serum-free cell culture medium developed for the cultivation of primary astrocytes from both neonatal and adult mouse and rat neural tissue.

Product data and images for AstroMACS Medium

Figures

Figure 1

**Superior astrocyte growth even with low seeding densities**

Figure 1

Superior astrocyte growth even with low seeding densities

Figure 2

Preservation of healthy astrocytes for downstream experiments

Figure 2

Preservation of healthy astrocytes for downstream experiments

Figure 3

**AstroMACS Separation Buffer removes dead cells and enhances adult astrocyte culture:**
Adult astrocytes were isolated from the brain of 8-week-old mice using the Adult Brain Dissociation Kit and the Anti-ACSA-2 MicroBead Kit in combination with (A) or without (B) the AstroMACS Separation Buffer.
Cells were then cultivated in AstroMACS Medium at a density of 100,000 cells /well in a 24-well Imaging Plate for 7 days. Cells were fixed and stained with Anti-GLAST antibody (green) and DAPI (blue).

Figure 3

AstroMACS Separation Buffer removes dead cells and enhances adult astrocyte culture:

Adult astrocytes were isolated from the brain of 8-week-old mice using the Adult Brain Dissociation Kit and the Anti-ACSA-2 MicroBead Kit in combination with (A) or without (B) the AstroMACS Separation Buffer.

Cells were then cultivated in AstroMACS Medium at a density of 100,000 cells /well in a 24-well Imaging Plate for 7 days. Cells were fixed and stained with Anti-GLAST antibody (green) and DAPI (blue).

Specifications for AstroMACS Medium

Overview

AstroMACS Medium is an optimized serum-free cell culture medium developed for the cultivation of primary astrocytes from both neonatal and adult mouse and rat neural tissue.

Detailed product information

Background information

AstroMACS Medium is an optimized serum-free cell culture medium for primary neonatal and adult mouse and rat astrocytes cultivation. It also allows cell survival and proliferation in a wide range of plating densities and is optimized for low-density plating of neonatal astrocytes. The medium ensures a high survival rate and viability of plated astrocytes, which can be used for different downstream applications.

Applications

Cultivation and proliferation of isolated primary neonatal astrocytes in a wide range of plating densities.
Cultivation of healthy adult astrocytes, together with AstroMACS Separation Buffer.

Resources for AstroMACS Medium

Documents and Protocols

Brochures/posters

Neuroscience - Inspiring technologies for creative scientists

Brochures/posters

AstroMACS Medium - For neonatal and adult primary astrocyte cultivation

Scientific posters

Highly efficient dissociation of distinct adult rodent brain regions ensures high cell viability and recovery for successful downstream processing

Protocols

Isolation of astrocytes from adult mouse brain

Data and images