The Naive CD4
+
T Cell Isolation Kit, mouse has been developed for the isolation of untouched naive CD4
+
T helper cells from suspensions of mouse spleen cells or lymph node cells.

Data and images for
Naive CD4
+
T Cell Isolation Kit
, mouse

Figures

Figure 1

Naive CD4
+
T cells were isolated from a single-cell suspension from mouse spleen using the Naive CD4
+
T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD45-VioGreen (# 130-102-412) CD4-VioBlue® (# 130-102-456), CD62L-PE (# 130-102-543), CD3ε-APC-Vio770™ (# 130-102-306), and CD44-FITC (# 130-102-511) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence and a gate was set on CD45
+
cells.
Before separation
Unseparated fraction
Gated on CD45
+
CD3ε
+
CD4
+
T cells
Isolated naive CD4+ T cells
Isolated naive CD4
+
cells
Gated on CD45
+
CD3ε
+
CD4
+
T cells

Specifications for
Naive CD4
+
T Cell Isolation Kit
, mouse

Overview

The Naive CD4
+
T Cell Isolation Kit, mouse has been developed for the isolation of untouched naive CD4
+
T helper cells from suspensions of mouse spleen cells or lymph node cells.

Detailed product information

Detailed separation procedure

Mouse naive CD4
+
T cells are isolated by depletion of memory CD4
+
T cells and non-CD4
+
T cells. Single-cell suspensions of mouse spleen or lymph nodes are indirectly magnetically labeled with a cocktail of biotinylated monoclonal antibodies, as primary labeling reagent, and Anti-Biotin MicroBeads, as secondary labeling reagent. Simultaneously, CD44 MicroBeads are added to label memory T cells. No washing steps are required during magnetic labeling. The magnetically labeled non-target cells are depleted by retaining them on a MACS
®
Column in the magnetic field of a MACS Separator, while the unlabeled target cells pass through the column.

Applications

Naïve CD4
+
T cells are perfect for use in
in vitro
analysis of T cell differentiation, adoptive transfer experiments, studies on signal requirements for CD4
+
T cell activation and proliferation, and for studies on regulation of CD4
+
T cell cytokine expression.

Columns

LS or autoMACS
®
Columns.

Resources for
Naive CD4
+
T Cell Isolation Kit
, mouse

Reviews for
Naive CD4
+
T Cell Isolation Kit
, mouse

Naive CD4+ T Cell Isolation Kit - Our Gold Standard

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Naive CD4+ T Cell Isolation Kit, mouse (130-104-453)

For a CD4 T cell lab, we use a lot of these kits. We've tried others, but this has always come out on top for quality.

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Naive CD4
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