Clone:
GK1.5
Type of antibody:
Primary antibodies
Isotype:
rat IgG2bκ
Applications:
FC, MICS, IF, IHC
Alternative names:
L3T4, Ly-4, Leu-3, T4

Extended validation for CD4 Antibody, anti-mouse

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD4. Splenocytes from BALB/c mice were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD4. Splenocytes from BALB/c mice were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD4. Splenocytes from BALB/c mice were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD4. Splenocytes from BALB/c mice were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD4. Splenocytes from BALB/c mice were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD4. Splenocytes from BALB/c mice were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD4. Splenocytes from BALB/c mice were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD4. Splenocytes from BALB/c mice were stained with CD4 antibodies and with a suitable counterstaining. As a control, CD4 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD4 (GK1.5). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD4 (GK1.5). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD4 (GK1.5). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD4 Antibody, anti-mouse

Overview

Clone GK1.5 recognizes the mouse CD4 (L3T4) antigen. In mice, CD4 is expressed on T helper cells, regulatory T cells, and at lower levels on subpopulations of NKT cells and dendritic cells. It is furthermore detected on most thymocytes (CD4
+
CD8
+
and CD4
+
CD8
thymocytes).

Alternative names

L3T4, Ly-4, Leu-3, T4

Detailed product information

Technical specifications

CloneGK1.5
Clonalitymonoclonal
Isotyperat IgG2bκ
Isotype controlIsotype Control Antibody, rat IgG2b
Hostrat
Type of antibodyPrimary antibodies
Speciesmouse
AntigenCD4
Alternative names of antigenL3T4, Ly-4, Leu-3, T4
Molecular mass of antigen [kDa]48
Distribution of antigendendritic cells, granulocytes, Langerhans cells, macrophages, monocytes, T cells, lymphocytes, neutrophils, T helper cells, thymocytes
Entrez Gene ID12504
RRIDAB_2727604, AB_2751634, AB_2751588, AB_2752205, AB_2752185, AB_2752219, AB_2819404, AB_2819535, AB_2819537, AB_2819676, AB_2659916, AB_2659917, AB_2727589

Resources for CD4 Antibody, anti-mouse

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