CD31 Antibody, anti-human

Name: CD31 Antibody, anti-human
Availability: InStock

CD31 Antibody, anti-humanExtended Validation

Clone:

AC128

Type of antibody:

Primary antibodies

Isotype:

mouse IgG1κ, mouse IgG1

Applications:

FC, MICS, IF, IHC, MC

Alternative names:

PECAM1, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, EndoCAM

Flow cytometry applications forCD31 Antibody, anti-human

APC
APC-Vio 770
Biotin
FITC
PE
PE-Vio 770
VioBlue

Conjugate:

APC

Recommended dilution:

1:50

Remark:

Cells should be stained prior to fixation, if formaldehyde is used as a fixative.

Tested:

QC tested

Reported:

25232467,

Products used:

130-119-976, 130-119-891

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:50)

Description:

Human peripheral blood mononuclear cells (PBMCs) were stained with CD31 antibodies or with the corresponding isotype control antibodies (left images) as well as with CD4 antibodies and analyzed by flow cytometry using the MACSQuant^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

MICS (MACSima Imaging Cyclic Staining) applications forCD31 Antibody, anti-human

APC
FITC
PE

Conjugate:

APC

Recommended dilution:

1:50

Fixation:

PFA

Tested:

during development

Species tested:

human

Products used:

130-119-976, 130-119-891

Compatible applications:

IF, IHC

Protocols:

Protocol for MICS experiments

Conjugate:

APC

Recommended dilution:

1:50

Fixation:

Acetone

Tested:

during development

Species tested:

human

Products used:

130-119-976, 130-119-891

Compatible applications:

IF, IHC

Protocols:

Protocol for MICS experiments

Mass cytometry applications forCD31 Antibody, anti-human

pure

Conjugate:

pure

Products used:

130-108-038

Extended validation for CD31 Antibody, anti-human

Specificity

Epitope competition

In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.

Other clones	Overlap in epitope recognition with AC128
MBC.78:2	-
L133.1	-
WM59	-
MEM-05	++
1F11	-
M89D3	-
REA1028	++
Cells were incubated with an excess of purified unconjugated CD31 (AC128) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for CD31. Human peripheral blood mononuclear cells (PBMCs) were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD31. Human peripheral blood mononuclear cells (PBMCs) were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD31. Human peripheral blood mononuclear cells (PBMCs) were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD31. Human peripheral blood mononuclear cells (PBMCs) were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD31. Human peripheral blood mononuclear cells (PBMCs) were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD31. Human peripheral blood mononuclear cells (PBMCs) were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD31. Human peripheral blood mononuclear cells (PBMCs) were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for CD31 Antibody, anti-human

Overview

CD31, a 120–140 kDa single-chain transmembrane glycoprotein, is present on virtually all monocytes, platelets, and granulocytes. CD31 is also expressed by lymphocyte subsets. During maturation of CD4

⁺

T cells, the expression of CD31 changes: CD4

⁺

recent thymic emigrants (RTEs) express CD31 in contrast to central naive T cells. CD31 is highly expressed on endothelial cells, especially at their juncture, and is also known EndoCAM.

Alternative names

PECAM1, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, EndoCAM

Detailed product information

Technical specifications

Clone	AC128
Clonality	monoclonal
Isotype	mouse IgG1κ, mouse IgG1
Isotype control	Isotype Control Antibody, mouse IgG1
Host	mouse
Type of antibody	Primary antibodies
Species	human, non-human primate
Cross-reactivity	rhesus monkey ( Macaca mulatta )
Antigen	CD31
Alternative names of antigen	PECAM1, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, EndoCAM
Molecular mass of antigen [kDa]	80
Distribution of antigen	endothelial cells, fibroblasts, granulocytes, macrophages, monocytes, NK cells, osteoclasts, platelets, T cells, leukocytes, lymphocytes, basophils, neutrophils, plasma cells, granulocytes, endothelial cells, fibroblasts, leukocytes, lymphocytes, macrophages, monocytes, NK cells, osteoclasts, platelets, T cells, basophils, neutrophils, plasma cells
Entrez Gene ID	5175
RRID	AB_2733637, AB_2733806, AB_2733807, AB_2784125, AB_2784124, AB_2784127, AB_2784126, AB_2751958, AB_2751911, AB_2751956, AB_2751909, AB_2751957, AB_2751910, AB_2660571, AB_2733636

Resources for CD31 Antibody, anti-human

Certificates

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to search for Certificates of Analysis (CoA) by lot number.

Reviews for CD31 Antibody, anti-human

Great CD31 Antibody from Miltenyi Biotec

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CD31-FITC, human (130-117-390)

This antibody gives specific signal on frozen-tissue sections with little background. Publishable results!

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References for CD31 Antibody, anti-human

Publications

Kimmig, S. et al. (2002) Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood. J. Exp. Med. 195: 789-794

Applications

Extended validation