mRNA transfection for stem cell differentiation and reprogramming

mRNA transfection is a powerful tool for modulating cell fate while eliminating the risk of insertional mutagenesis. Using simple lipid-based transfection, modified mRNAs are delivered into the cell allowing high-level, transient expression of key developmental regulators, recombinases, or markers. This pioneering non-integrative mRNA technology has revolutionized reprogramming of somatic cells and offers new approaches for direct programming and differentiation of stem cells.

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