Enrichment of CD138+ plasma cells for analysis of Multiple Myeloma samples

  • Direct isolation of CD138+ PCs from bone marrow or peripheral blood in 30 minutes 
  • No density gradient centrifugation, no erythrocyte lysis 
  • Scalable automation solutions 

MACSprep Multiple Myeloma CD138 MicroBeads, human allow the isolation of CD138+ plasma cells (PCs) directly from bone marrow or peripheral blood without the need for gradient centrifugation or erythrocyte lysis. The minimal manipulation of sample ensures high integrity of CD138+ PCs, suitable for further cellular (flow cytometry), cytogenetic (FISH), or molecular analysis (PCR, microarray) of bone marrow samples.

Basic principle

Basic principle of MACSprep™ Technology
View details

Three easy steps to isolate PCs directly from peripheral blood or bone marrow samples: 

1. Magnetic labeling: PCs are magnetically labeled directly in the sample with MACSprep Chimerism Microbeads.  

2. Magnetic separation: The flow-through fraction, containing erythrocytes and non-target cells, can be collected as the negative fraction.  

3. Elution of labeled cells: The column is removed from the separator. The retained cells are eluted as the enriched, positively selected PCs. 

Data

Isolation of CD138+ PCs from bone  marrow

CD138+ PCs were isolated from a blood marrow sample using MACSprep™ Multiple Myeloma CD138 MicroBeads and the autoMACS® Pro Separator.  

Before separation
After separation

Cells were fluorescently stained with CD138-APC, CD38-FITC, and CD45-VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Video

video preview Play video

Video
Direct cell isolation from blood or bone marrow on the  autoMACS® Pro Separator

With the autoMACS® Pro Separator, CD138+ PCs can be automatically isolated from peripheral blood or bone marrow samples. Take this short instrument tour and see how your target cells are being separated without the need for density gradient centrifugation or red blood cell lysis.

Resources

Download PDF

Brochure 
MACSprep Multiple Myeloma CD138 MicroBeads – Direct plasma cell isolation from bone marrow 
 

Download PDF

Application note 
Saving valuable hands-on time – automated CD138+ plasma cell enrichment 
 

Related information

Matching products: