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As a cytometry specialist, I often encounter misconceptions when working in the field. Assumptions are made about excitation/emission patterns that can be very problematic. For example, using a 488 nm laser to excite PerCP-Cy™ 5.5 and working with a spatially separated laser configuration, a user might assume they will only detect light in channels associated with the 488 nm excitation ranges. This is actually false as this protein is excited by the 635 nm laser and is shown in the figure below.
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