StraightFrom
®
Whole Blood CD8 MicroBeads were developed for rapid positive selection of CD8
+
cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

Data and images for
StraightFrom
®
Whole Blood CD8 MicroBeads
, human

Figures

Figure 1

Separation of a whole blood sample using the StraightFrom
®
Whole Blood CD8 MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Unlysed cells were fluorescently stained with CD3-PE, CD8‑APC, as well as CD45‑VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
After separation
View details

Figure 1

Separation of a whole blood sample using the StraightFrom
®
Whole Blood CD8 MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Unlysed cells were fluorescently stained with CD3-PE, CD8‑APC, as well as CD45‑VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Separation of a whole blood sample using the StraightFrom
®
Whole Blood CD8 MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Unlysed cells were fluorescently stained with CD3-PE, CD8‑APC, as well as CD45‑VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for
StraightFrom
®
Whole Blood CD8 MicroBeads
, human

Overview

StraightFrom
®
Whole Blood CD8 MicroBeads were developed for rapid positive selection of CD8
+
cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

Detailed product information

Background information

In whole blood the CD8 antigen is expressed on human cytotoxic T cells and on a subset of CD16
+
NK cells. The CD8 antigen acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8
+
cytotoxic T cells.

Detailed separation procedure

Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom
®
Whole Blood CD8 MicroBeads to be subsequently separated with the autoMACS
®
Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Downstream applications

StraightFrom
®
Whole Blood MicroBeads allow the fast isolation of CD8
+
cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. StraightFrom Whole Blood MicroBeads in combination with the autoMACS
®
Pro Separators allow standardization of the cell separation procedure and safe handling of hazardous samples
1
. The purified CD8
+
cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation
2,3
.

Columns

autoMACS
®
Columns or Whole Blood Columns.

Resources for
StraightFrom
®
Whole Blood CD8 MicroBeads
, human

References for
StraightFrom
®
Whole Blood CD8 MicroBeads
, human

Publications

  1. Meyer, R. G. et al. (2007) Prophylactic transfer of CD8-depleted donor lymphocytes after T-cell-depleted reduced-intensity transplantation. Blood 109: 374-382
  2. Friedrich, T. C. et al. (2004) Consequences of cytotoxic T-lymphocyte escape: common escape mutations in simian immunodeficiency virus are poorly recognized in naive hosts. J. Virol. 78: 10064-10073
  3. Koehl, U. et al. (2003) Quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection: the Frankfurt experience. Leukemia 17: 232-236
Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

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®
Whole Blood CD8 MicroBeads
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