Clone:
AF12-7H3
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC, MICS, IF, IHC, ICC
Alternative names:
NCAM1, MSK39, NCAM, Leu-19, NKH-1

Extended validation for CD56 Antibody, anti-human

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD56. Human peripheral blood mononuclear cells (PBMCs) were stained with CD56 antibodies and with a suitable counterstaining. As a control, CD56 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD56. Human peripheral blood mononuclear cells (PBMCs) were stained with CD56 antibodies and with a suitable counterstaining. As a control, CD56 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD56. Human peripheral blood mononuclear cells (PBMCs) were stained with CD56 antibodies and with a suitable counterstaining. As a control, CD56 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD56. Human peripheral blood mononuclear cells (PBMCs) were stained with CD56 antibodies and with a suitable counterstaining. As a control, CD56 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD56. Human peripheral blood mononuclear cells (PBMCs) were stained with CD56 antibodies and with a suitable counterstaining. As a control, CD56 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD56. Human peripheral blood mononuclear cells (PBMCs) were stained with CD56 antibodies and with a suitable counterstaining. As a control, CD56 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD56. Human peripheral blood mononuclear cells (PBMCs) were stained with CD56 antibodies and with a suitable counterstaining. As a control, CD56 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD56. Human peripheral blood mononuclear cells (PBMCs) were stained with CD56 antibodies and with a suitable counterstaining. As a control, CD56 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD56. Human peripheral blood mononuclear cells (PBMCs) were stained with CD56 antibodies and with a suitable counterstaining. As a control, CD56 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD56. Human peripheral blood mononuclear cells (PBMCs) were stained with CD56 antibodies and with a suitable counterstaining. As a control, CD56 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD56. Human peripheral blood mononuclear cells (PBMCs) were stained with CD56 antibodies and with a suitable counterstaining. As a control, CD56 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD56. Human peripheral blood mononuclear cells (PBMCs) were stained with CD56 antibodies and with a suitable counterstaining. As a control, CD56 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD56 (AF12-7H3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD56 (AF12-7H3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD56 (AF12-7H3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD56 Antibody, anti-human

Overview

Clone AF12-7H3 recognizes the human CD56 antigen, a glycoprotein of the Ig-superfamily also known as neural cell adhesion molecule (NCAM), which is expressed in blood on practically all resting and activated NK cells and on a minor subset of CD3
+
T cells. CD56 is reported to be expressed on rhesus monkey monocytes but not on NK cells.
CD56 is also expressed in brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal-derived tumors, myelomas, and myeloid leukemias also express CD56.
The monoclonal antibody AF12-7H3 recognizes an epitope distinct from those recognized by the CD56-specific monoclonal antibodies NCAM16.2 and B159.

Alternative names

NCAM1, MSK39, NCAM, Leu-19, NKH-1

Detailed product information

Technical specifications

CloneAF12-7H3
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
AntigenCD56
Alternative names of antigenNCAM1, MSK39, NCAM, Leu-19, NKH-1
Molecular mass of antigen [kDa]92
Distribution of antigenmonocytes, NK cells, ILC, T cells, leukemia cells, ES and iPS cells, brain, cardiac muscle, liver, lung, skeletal muscle, smooth muscle, spleen
Entrez Gene ID4684
RRIDAB_2726087, AB_2733824, AB_2733825, AB_2726361, AB_2726084, AB_2726363, AB_2726086, AB_2726362, AB_2726085, AB_2726364

Reviews for CD56 Antibody, anti-human

Staining Peripheral Blood with Anti-CD56 PE

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CD56-PE, human (130-113-307)

Works well. Reproducible results.

Staining Peripheral Blood with Anti-CD56 PE

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CD56-PE, human (130-113-869)

Works well. Reproducible results.

References for CD56 Antibody, anti-human

Publications

  1. Carter, D. L. et al. (1999)
    CD56 identifies monocytes and not natural killer cells in
    Rhesus macaques
    .
    Cytometry 37: 41-50
  2. Meng, J. et al. (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice. PLoS One 6(3): e17454
  3. Stockholm, D. et al. (2011) Bistable cell fate specification as a result of stochastic fluctuations and collective spatial cell behaviour. PLoS One 5(12): e14441

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