StemMACS™ Lin28 mRNA encodes the human RNA-binding protein Lin28 and is designed for transient transfection.

Data and images for StemMACS™ Lin28 mRNA, human

Figures

Figure 1

Lin28 expression after mRNA transfection:
Human BJ fibroblasts were transfected once with 150 ng StemMACS™ Lin28 mRNA. 24 hours after transfection, Lin28 expression (magenta) and cytoplamic localization (DAPI counterstain) were analyzed by immunofluorescence microscopy.
Untransfected cells
Lin28-transfected cells
View details

Figure 1

Lin28 expression after mRNA transfection:
Human BJ fibroblasts were transfected once with 150 ng StemMACS™ Lin28 mRNA. 24 hours after transfection, Lin28 expression (magenta) and cytoplamic localization (DAPI counterstain) were analyzed by immunofluorescence microscopy.
View details

Figure 1

Lin28 expression after mRNA transfection:
Human BJ fibroblasts were transfected once with 150 ng StemMACS™ Lin28 mRNA. 24 hours after transfection, Lin28 expression (magenta) and cytoplamic localization (DAPI counterstain) were analyzed by immunofluorescence microscopy.

Specifications for StemMACS™ Lin28 mRNA, human

Overview

StemMACS™ Lin28 mRNA encodes the human RNA-binding protein Lin28 and is designed for transient transfection.

Applications

  • Modulation of pluripotency
  • Reprogramming

Detailed product information

Background information

The RNA binding protein Lin28 is a conserved regulator of cell fate. In vertebrates, Lin28 promotes pluripotency and controls differentiation in diverse tissues. Lin28 can directly inhibit let-7 microRNA processing. It is highly expressed in human embryonic stem cells and has been used to enhance the generation of induced pluripotent stem cells.

Detailed procedure

For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Quality description

StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.

Related products for
StemMACS™ Lin28 mRNA, human

6 products available | view all